慢性乙型肝炎患者体内一氧化氮和一氧化氮合酶水平的研究

Nitric oxide and nitric oxide synthase in patients with chronic hepatitis B

目的 探讨慢性乙型肝炎(慢乙肝)患者体内一氧化氮(NO)和一氧化氮合酶(NOS)水平及其意义。方法 检测37例慢乙肝患者NO、NOS[诱生型NOS(iNOS)和结构型NOS(cNOS) ]、肝功能、乙型肝炎病毒(HBV)DNA和HBV基因型(GT)并做出统计分析。结果 慢乙肝患者组与正常对照组比较,NO和iNOS的浓度均明显升高(P <0 0 5 ) ;丙氨酸转氨酶(ALT)异常组与正常对照组及ALT正常组比较:NO和iNOS的浓度均明显升高(P <0 0 5 ) ;ALT正常组与正常对照组比较:NO的浓度明显升高(P <0 0 5 ) ;cNOS在各组间比较差异无统计学意义。在慢乙肝患者中:NO和iNOS浓度与ALT水平呈明显正相关(r=0 36 7,r=0 4 74 )。NO和NOS与HBVDNA指标间均无明显相关关系。不同基因型组之间,NO和NOS的浓度差异无统计学意义(P >0 0 5 )。结论 在慢乙肝患者中,存在NO和iNOS水平升高的现象。慢乙肝患者ALT升高时,NO浓度高,对机体损伤重;慢乙肝患者ALT正常者,NO对机体无明显的损伤。NO和NOS与HBVDNA是相对独立的检测指标。NO水平与不同HBVGT患者病情及预后不同无明显关系。

Objective To investigate nitric oxide(NO) and nitric oxide synthase(NOS) in patients with chronic hepatitis B. Methods Nitric oxide and nitric oxide synthase, including inducible NOS (iNOS) and constitutive NOS (cNOS), were measured in patients and control groups, then were statistically analyzed. Results NO and iNOS were significantly higher in patients with hepatitis B than in the controls (P<0.05). NO and iNOS were significantly higher in patients with increased ALT than in the controls and in patients with normal ALT(P<0.05). NO was significantly higher in patients with normal ALT than in the controls (P<0.05). cNOS were not significant different among these groups. NO and iNOS significantly correlated with ALT in patients with hepatitis B(r=0.367 and r=0.474). No significant relationship was found among NO, NOS and HBV DNA. Among different genotype groups, NO and NOS had no significant difference. Conclusion NO and NOS were higher in patents with chronic hepatitis B. In patients with increased ALT, NO's damage was severe. In patients with normal ALT, there was no significant damage caused by NO. NO should be detected in patients with hepatitis B in addition to HBV markers.