DGGE污泥堆肥工艺微生物种群结构分析

Analysis of microbial community structure with DGGE sludge compost technology.

用DGGE指纹图谱技术对污泥堆肥工艺中的细菌种群动态变化及多样性进行了研究.结果表明,生物法污泥堆肥周期小于8d.对污泥堆肥各工艺环节样品进行DGGE指纹图谱和相似性系数Cs值分析,发现随着反应的持续进行,微生态结构的Cs值越来越高,说明微生物种群结构愈趋稳定.证实污泥微生态能迅速进行优胜劣汰的筛选,调整内部细菌种群结构,从而达到适应环境的目的.在发酵过程中形成的优势细菌种群能长时间保持稳定.

The dynamic change and diversity of bacterial community in sludge compost technology was studied using DGGE fingerprint atlas technique. The period of sludge composting process with biological technique was less than 8 days. The DGGE fingerprint atlas and pairwise similarity coefficient Cs value were analyzed for the sample of each technologic link in sludge compost. It was discovered that Cs value of microecological structure was higher and higher with the reaction in progress continuously, showing the microbial community structure being more and more stable. It was confirmed that the sludge microecology could rapidly screen out the priorities and discard off the inferior, adjusting the internal bacterial community structure, thus reaching the purpose of adaptable environment. The priorities formed in the fermentation process could maintain stability for a long time.