放射线照射后NK-92细胞对人宫颈癌细胞体外杀伤活性的研究

In Vitro Cytotoxic Activity of Irradiated NK-92 Cells against Human Cervical Cancer Cells

目的研究放射线照射后NK-92细胞对人宫颈癌细胞的体外杀伤活性.方法应用医用电子直线加速器对NK-92细胞进行照射处理(0、400cGy)后,以体外培养人宫颈癌细胞系Hela为靶细胞,以NK-92的敏感细胞株K562作为对照,应用4h51Cr释放法检测不同效靶比情况下NK-92细胞的杀伤活性.结果 NK-92细胞0 cGy照射组,效靶比较低时(1∶1 、5∶1) 对Hela细胞杀伤率为15%～33% ,随效靶比升高,杀伤率随之上升,10∶1 时杀伤率显著上升为47%, 20∶1时杀伤率仅上升了3%;对于K562细胞,杀伤率为33%～69%.400cGy 组照射后2d , NK-92细胞对Hela细胞不同效靶比的杀伤率与0cGy 组相比稍有降低,其总体杀伤率为14%～47%,对K562 细胞杀伤范围在30%～60%之间.结论放射线照射后的NK-92 细胞对人宫颈癌Hela细胞系具有一定的杀伤活性.

Objective To study in vitro cytotoxic activity of NK-92 cells against human cervical cancer cells and effect of irradiation on NK-92 cytotoxicity.Methods NK-92 cells were mixed with 51 Cr-labeled Hela or K562 cells at different effector-to-target ratios for a 4h 51 Cr-release assay. On the other hand, NK-92 cells were irradiated at 0 and 400 cGy and tested after 2d for cytotoxicity against K562 and Hela cells.Results NK-92 cells (0 cGy) displayed high in vitro cytotoxicity against Hela with a mean specific lysis of 47% and 50% at 10∶1 and 20∶1 E∶T ratios , while these levels at 1∶1 (15 %) and 5∶1 (33 %) E∶T ratios were lower; The cytotoxicity of NK-92 against K562 ranged from 33 % to 69 %. NK–92 cells maintained up to 14 %～47 % cytotoxicity against Hela and 30%～60% cytotoxicity against K562 when irradiated with 400cGy after 2d.Conclusion In vitro cytotoxic activity of NK-92 cells against Hela cells is high at suitable E∶T ratios,and substantial cytotoxicity can be retained up to irradiation dose of 400cGy.