CK在恶性黑色素瘤中的表达

Expression of Cell Keratin in Malignant Melanoma

目的检测CK在恶性黑色素瘤中的表达情况及意义。方法应用免疫组化PV-9000二步法对18例恶性黑色素瘤进行CK、Vimentin、S-100、HMB45的检测。结果在18例恶黑中CK的阳性表达率为67%(12/18);其中原发性恶黑16例,CK和Vimentin的表达率分别为63%(10/16)、100%(16/16);CK与S-100,CK与HMB45两项均呈阳性表达者各占50%(8/16),以上四项抗体均为阳性的表达率为44%(7/16);两例转移性恶黑四项抗体均为阳性表达,占100%(2/2)。结论CK在原发性和转移性恶黑中均有不同程度的阳性表达。

Objective To detect the expression and significance of cell keratin (CK) in malignant melanoma. Methods To detect CK、Vimentin、S-100、HMB_ 45 in 18 cases of malignant melanoma using immunohistochemical method—PV-9000 two steps. Results The positive expression rates of CK in 18 cases of malignant melanoma were 67%(12/18);among them, the positive rates of CK and Vimentin in 16 cases of primary melanoma were 63%(10/16),100%(16/16) respectively ;the positive rates CK and S-100 、CK and HMB_ 45 were 50%(8/16) respectively ,the positive expression rate of the above four kinds of antibodies was 44%(7/16); the positive expression rates of the four kinds of antibodies in two cases of metastatic melanoma were 100%(2/2).Conclusion There was positive expression in varying degrees of CK in primary and metastasis melanoma.