锌调控神经细胞生长抑素mRNA表达的研究

STUDY ON ZINC MODULATION OF SOMATOSTATIN mRNA IN NEURONS

目的:探讨锌对神经细胞生长抑素mRNA表达的影响。方法:利用不同含量锌的饲料饲喂生长大鼠及用不同含量锌的培养基培养神经-肿瘤杂交细胞株LA-N-5及LA-N-5-2,采用放射性同位素标记的生长抑素基因探针与脑组织细胞总RNA进行点杂交法检测不同的锌处理对大脑皮层以及培养的细胞株生长抑素基因表达的影响。结果:饲料缺锌的大鼠大脑皮层神经细胞生长抑素基因的表达较对喂组明显降低,补锌后可逆转。同时富锌培养的细胞较正常含锌培养的细胞生长抑素基因的转录有增高趋势。结论:锌可以在转录水平上影响神经细胞生长抑素基因的表达。

Objective: To study the effect of zinc on somatostatin mRNA (SS mRNA)content in neurons. Methods: Different zinc contents of feed and culture medium were separately used to feed rats and to culture LA-N-5 and LA-N-5-2 (neuro-tumor cells). Dot blot hybrid was used to determine SS mRNA content of cerebrum cortex in rats and cultured cells. Results: SS mRNA content of cerebrum cortex in zinc deficient rats was decreased, and zinc supplement could reverse the change. Moreover SS mRNA in cells cultured in rich zinc medium is higher than that in cells cultured in normal medium. Conclusion: Zinc can modulate expression of somatostatin gene in neuron.