血管紧张素转换酶基因的插入/缺失多态性在不同种族中老年人群中的分布

Distribution of insertion/deletion polymorphism of angiotensin I converting enzyme gene in mid-aged population of different races

目的:探讨血管紧张素转换酶(angiotensinIconvertingenzymeACE)基因内含子16中的插入/缺失(I/D)多态性在不同种族中老年人群之间的相互关系。方法:运用聚合酶链反应(PCR)、电泳分型等方法,检测美国黑人(89例)、美国白人(47例)、墨西哥人(425例))和中国佳木斯地区汉族人(107例)的等位基因频率和基因型频率。结果:在不同种族中老年人群中II、ID、DD基因型频率分别为:美国白人:0.170、0.447、0.383;美国黑人:0.180、0.483、0.337;墨西哥人:0.297、0.463、0.240;中国佳木斯地区汉族人:0.392、0.430、0.178。等位基因I、D频率分别为:美国白人:0.394、0.606;美国黑人:0.421、0.579;墨西哥人:0.528、0.472;中国佳木斯地区汉族人:0.607、0.393(并与文献报道的关于日本、挪威、法国和意大利的结果相比较)。结果显示,中国佳木斯地区汉族人群的ACE基因IN16(I/D),D等位基因频率与墨西哥人和日本人差异不显著(P>0.05),而与美国黑人、美国白人、挪威人、法国人和意大利人差异极其显著(P<0.01)。结论:结果提示,ACE基因的多态性位点在不同种族中老年人群中存在差异。

Objective: To investigate the relations of the insertion/deletion polymorphism of angiotensin I converting enzyme gene in mid-aged population of different races.Methods: Polymerase chain reaction(PCR) and electrophoresis were used to determine the allele frequencies and genotype frequencies in an 89 black American population, a 47 white American population, a 425 Mexican population and a 107 Chinese han population of Jiamusi. Results: The occurrence frequency of ACE genotype in mid-aged population of different races was respectively as follow: II 0.170,ID 0.447,DD 0.383 in white American; II 0.180, ID 0.483,DD 0.337 in black American; II 0.297,ID 0.463, DD 0.240 in Mexican; II 0.392,ID 0.430,DD 0.178 in Chinese han of Jiamusi. The frepuency of I and D alleles was 0.394 and 0.606 in white American; 0.421 and 0.579 in black American; 0.528 and 0.472 in Mexican; 0.607and 0.393 in Chinese han of Jiamusi. The results showed that the deletion allelic frequency of ACE gene was similar in Chinese han of Jiamusi with Mexican and Japanese, but different from white American, black. American,Norwegian,French and Italian. Conclusion: There are differences in mid-aged population of different races in the distribution of the ACE gene polymorphism sites.