人脐血造血祖细胞体外培养的研究

STUDIES ON HEMAPOIETIC PROGENITOR CELLS IN HUMAN UMBILICAL CORD BLOOD IN VITRO CULTURE

应用体外培养方法对人脐血造血祖细胞（ＣＦＵ－ＧＭ、ＣＦＵ－Ｅ、ＢＦＵ－Ｅ、ＣＦＵ－Ｆ）数量及其特征进行观察，并与成人外周血及骨髓相比较。结果显示：①脐血中ＣＦＵ－ＧＭ、ＣＦＵ－Ｅ、ＢＦＵ－Ｅ数量约相当于成人外周血的７～１８倍；ＣＦＵ－ＧＭ及ＣＦＵ－Ｅ数量与骨髓相比无统计学差异，但ＢＦＵ－Ｅ低于骨髓；②脐血中ＣＦＵ－ＧＭ的集落大小、细胞构成、集落生长曲线明显不同于成人外周血及骨髓；③脐血红系祖细胞生长模式近似于骨髓；④脐血中可培养出ＣＦＵ－Ｆ，其数量约为骨髓的２／５，而外周血中则无ＣＦＵ－Ｆ形成。提示人脐血中富含造血干／祖细胞，而且在数量和特征上明显不同于外周血和骨髓，在移植中可能更具有重建骨髓造血的能力。

The numbers and their behaviour of human hemapoietic progenitor cells in um- bilical cord blood(UCB) marrow(BM)were observed by the method of cell culture in vitroand capability of UCB as a source of transplantable stem/progenitor cells, compared withadult PB and BM, was evaluated.The results showed :①The numbers of CFU- GM 、CFU-E、BFU- E in UCB and BM were significantly higher than that of PB,about 7～18 times,there was no statistical difference in the number of CFU-GM,CFU-E between UCB andBM,but the number of BFU-E in UCB was lower than that of BM;②The differences ofCFU- GM colony size,composition and colony growth time curve in UCB,PB and BMwere found;③The growth pattern of CFU- E,BFU- E was similar to BM;④CFU- Fcolonies were observed in UCB and the numbers of them were lower than that of BM, about two fifths in comparison with bone marrow,no colony was found in PB. All thesedata suggested that human umbilical cord blood was rich in hematopioetic stem/progen-iter cells and there were obvious different numbers and behaviour among the progenitorcells in UCB, PB and BM, which the capability for the restoration of human bone marrowfunction might be stronger than that of PB and BM.