Endostatin基因mRNA在卵巢上皮癌中的表达及其临床意义

Expression and clinical significance of endostatin gene mRNA in human ovarian cancer

目的探讨Endostatin基因mRNA在卵巢上皮癌中的表达以及临床意义。方法应用逆转录聚合酶链反应技术检测48例卵巢上皮癌组织和18例正常卵巢组织中Endostatin基因的表达。结果①卵巢癌组织中Endostatin基因的表达(平均0.525;范围0.09～1.31)明显高于正常卵巢组织(平均0.28;范围0.08～0.38;P<0.05)。②Endostatin基因在晚期(Ⅲ～Ⅳ期)上皮癌组织中的表达明显高于早期(Ⅰ～Ⅱ期)上皮癌(P<0.05),而与年龄、病理类型、病理分级无关。结论Endostatin基因在卵巢癌上皮癌组织中的表达比正常组织表达高,在晚期卵巢癌上皮癌组织中的表达比早期高。本实验结果为上皮性卵巢癌的生物治疗提供了新的治疗策略。

Objective To study expression and clinical meaning of endostatin gene mRNA in epithelial ovarian cancer.Methods Expression of endostatin gene was evaluated by reverse transcription polymerse chain reaction (RT PCR) in 48 cases of epithelial ovarian cancer and that in 18 cases of normal tissue.Results ①Endostatin gene expression in 48 cases of ovarian cancer(median 0.525;range 0.09～1.31) was significantly higher than that in 18 cases of normal tissue(median 0.28;range 0.08～0.38; P <0.05).②Expression of endostatin in ovarian cancer was much higher in advanced stage than that in early stage( P <0.05),but was not related to patient age at diagnosis,histological type and histological grade( P >0.05).Conclusion Expression of endostatin gene is much higher in malignant and advanced stage of epithelial ovarian cancer.Our results may lead to the development of a new therapeutic strategy for epithelial ovarian cancer.