摘要
目的通过检测内皮素1(ET1)在牙髓组织中的表达,探讨ET1与正常及炎性牙髓组织的关系。方法以改良的单纯穿髓开放法建立Wistar大鼠实验性牙髓炎的动物模型,病理观察分析该型的牙髓组织情况;Northern杂交分析牙髓组织内ET1的mRNA表达水平。结果ET1mRNA在正常组及牙髓炎组均有表达,且在炎症时增长明显,以牙髓炎3天组表达量最多。结论ET1参与了牙髓炎症的发生与发展过程。
Objective To study the relationship between the expression of ET 1 mRNA and pulpal tissue inflammation.Methods The experimental pulpitis models of rat were induced through simply pulp exposure by drilling maxillary molar with cool water.Pulpitis were observed pathologically;The expression of ET 1 mRNA in pulp were analyzed by Northern hybridization.Results ET 1 expression was observed in normal and inflammatory pulp and was up regulated with its dominance at day 3.Conclusion ET 1 may be involved in the occurrence and development of pulpitis.
出处
《哈尔滨医科大学学报》
CAS
北大核心
2005年第3期236-239,共4页
Journal of Harbin Medical University
基金
黑龙江省科技攻关项目资助(GC02C14902)
