梅毒患者外周血树突状细胞亚型的表达

Dendritic Cells Subsets in Peripheral Blood of Patients with Syphilis

目的通过检测梅毒患者外周血树突状细胞(DC)各亚型的表达,分析其与快速血浆反应素实验(RPR)滴度的相关关系,了解树突状细胞在梅毒的发病机制中的作用。方法应用流式细胞仪测定23例梅毒患者和17例正常人的外周血DC各亚型的表达;RPR检测梅毒患者血清抗心磷脂抗体。结果23例梅毒患者外周血髓系树突状细胞亚型(CD11c+)的表达为(1.51±2.04)%,与17例正常对照组(0.37±0.17)%比较显著升高(P<0.01),梅毒患者外周血淋巴系树突状细胞亚型(CD123+)的表达为(0.37±0.21)%,与正常对照组(0.39±0.21)%比较无显著差异(P>0.05)。梅毒患者泛发皮疹组与非泛发皮疹组比较,CD11c+的表达分别为(1.28%±1.93)%和(1.94±2.31)%,CD123+的表达分别为(0.33±0.21)%和(0.44±0.21)%,两组间无显著差异(P>0.05)。CD11c+及CD123+的表达与RPR滴度不存在相关关系(r分别为-0.15和0.32,P均>0.05)。结论梅毒螺旋体进入体内后主要诱导CD11c+型DC表达,引发Th1反应,启动细胞免疫;DC各亚型的表达与梅毒患者皮疹面积以及病情的活动程度无相关关系。

Objective To investigate the role of dendritic cells (DC) in the pathogenesis of syphilis. The DC subsets in peripheral blood were detected in patients with syphilis, and the correlation between the expression of DC subsets and the titer of rapid plasma reagin test (RPR) was analyzed. Methods Flow cytometer were used to detect DC subsets in peripheral blood in 23 syphilis patients and 17 healthy controls, and RPR were used to detect serum anticardiolipin antibodies in 23 syphilis patients. Results The expression of CD11c+ DC in peripheral blood was (1.51±2.04)% in syphilis patients. Compared with 17 normal controls (0.37±0.17)%, it was increased sianificantly(P<0.05). The expression of CD123+ DC in peripheral blood in syphilis was (0.37±0.21)%. Compared with normal controls (0.39±0.21)%, they had no significant differences(P>0.05). In syphilis patients with generalized lesions and with non-generalized lesions, the expression of CD11c+ DC were(1.28±1.93)% and (1.94 ±2.31)% respectively, and that of CD123+ DC were (0.33 ±0.21)% and (0.44 ±0.21)% respectively, there was no significant difference(P>0.05) and no correlation between expression of CD11c+ DC or CD123+ DC and the titer of RPR. (r=-0.15 and 0.32, P>0.05). Conclusion Treponema pallidum mainly induced the expression of CD11c+ DC and initiated cell-mediated immune response. And the expression of CD11c+ DC had no correlation with the area involved or the activity of disease.