视神经离断后坐骨神经移植修复反应的初步研究

Sciatic nerve transplantation and optic nerve regeneration

目的建立大鼠坐骨神经支持下的视神经再生模型。方法大鼠右侧视神经离断后移植一段自身视神经或坐骨神经,分别建立视神经自身吻合模型和视神经-坐骨神经吻合模型。建立模型后第3天、第7天和第14天处死动物,处死前3d将荧光染料Dil注射到移植的神经断端,应用逆行标记的方法观察不同模型中视网膜神经节细胞(RGCs)轴突的再生情况。结果术后3d,两组均无明显的Dil标记细胞;7d后,视神经自身吻合组无明显的荧光标记细胞,视神经-坐骨神经吻合组可见明显的Dil标记细胞,细胞密度分别为(152±26)/mm2,14d后增加为(297±31)/mm2,而此时,视神经自身吻合组仅在一只大鼠的视网膜铺片上见到一Dil标记细胞。结论成功建立了坐骨神经支持下的视神经再生模型。

Objective To establish a regeneration model of the optic nerve in rats.Methods After the right optic nerve was transected intraorbitally,an optic nerve or sciatic nerve graft was sutured to the axotomized optic nerve. Animals were sacrificed at 3 days,7 days or 14 days after undergoing the procedure. Regenerating RGCs were labeled with the dye Dil at the distal end of the optic or peripheral nerve graft 3 days before the animals were sacrificed.Results Three days after surgery,no Dil-labeled RGCs were found in any group. Seven days later,a number of Dil-labeled cells were found in the sciatic-optic nerve aastomosis group. Cell densities were (152±26)mm2 and increased to (297±31)mm2 14 days later. However,only one Dil-labeled cell was found in the self-aastomosis group 14 days later,while no cells were found 7 days later.Conclusion A regeneration model of the optic nerve was successfully established.