人乳头瘤病毒与结直肠癌发生和病理特征的关系

The relationship between human papillomavirus infection and the carcinogenesis and pathological features of colorectal carcinoma

目的检测和比较人结直肠癌组织和邻近正常结直肠黏膜组织中人乳头瘤病毒(HPV)的感染率和亚型分布状况,研究HPV与人结直肠癌的关系。方法运用聚合酶链式反应(PCR)检测72例结直肠癌患者手术切除标本中的HPVL1基因,结果阳性的标本采用多重引物PCR和DNA原位杂交法进行HPV的亚型(包括HPV6、11、16、18)鉴定。结果72例肿瘤组织中有24例HPVL1基因阳性(33.3%);正常结直肠黏膜中均未检测到HPVL1基因。HPV感染与结直肠癌的发生部位、浸润深度和远处转移等临床病理因素相关。24例HPV阳性的结直肠癌标本中,14例为HPV16型(58.33%);2例为HPV18型(8.33%);8例分型不明确;无HPV6和HPV11感染。结论人乳头瘤病毒感染有可能与部分结直肠癌的发生、发展相关,其中以肿瘤高危型HPV(特别是HPV16型)的作用尤为重要。

Objective To investigate the relationship between HPV infection and human colorectal carcinogenesis. Methods Colorectal carcinoma specimens from 72 Chinese patients were studied. DNA extracted from colorectal tissue was screened for HPV L1 by polymerase chain reaction (PCR), HPV subtype 6,11, 16, and 18 were detected by PCR using specific primers and in situ hybridization using specific probe. Results Twenty-four specimens out of 72 (33%) colorectal cancer were HPV L1 positive. The normal colorectal mucosa was HPV L1 negative. The location, infiltration and metastasis of colorectal carcinoma were all significantly related with HPV infection. The predominant HPV subtype was HPV 16,which was found in 58% of all HPV-positive colorectal carcinoma. Conclusion The presence of HPV DNA suggests that HPV may be involved in the carcinogenesis of colorectal carcinoma. HPV infection is closely related with the malignant potential of colorectal cancer.