利用PCC技术预测γ射线对肝癌细胞的辐射效应

Predictive Assay of Radiant Response of Hepatoma Cells Exposed to γ-rays Measured by PCC Technique

利用早熟染色体凝集技术预测研究了γ射线对肝癌细胞SMMC7721的辐射效应。结果表明,G1和G2期细胞内的染色单体和等点染色单体断裂数与照射剂量之间存在线性相关性,染色单体断裂总数与细胞存活率之间存在良好的线性相关性。说明辐射诱导的染色单体断裂可以作为预测SMMC7721细胞内在辐射敏感性的指标,也可为临床诊断和治疗肝癌提供依据。

We investigate the radiation response of hepatoma cells SMMC-7721 by premature chromosome condensation (PCC) technique. A dose-dependent increase in radiation-induced chromatid/isochromatid breaks was observed in G1 and G2 phase respectively. A good relationship was found between cell survival and chromatin breaks. The resoults implied that low LET radiation-induced chromatid/isochromatid breaks can be possibly used as a good predictor of radiosensitivity of SMMC-7721 hepatoma cells, also it could be used to design radiotherapy schedule for liver cancer patients.