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Analysis of phosphate-accumulating organisms cultivated under different carbon sources with polymerase chain reaction-denaturing gradient gel electrophoresis assay 被引量:6

Analysis of phosphate-accumulating organisms cultivated under different carbon sources with polymerase chain reaction-denaturing gradient gel electrophoresis assay
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摘要 To investigate the microbial communities of microorganisms cultivated under different carbon sources, three sequencing batch reactors were operated. They were supplied with sewage, glucose and sodium acetate as carbon sources respectively and showed high phosphorus removal performance. The results of denaturing gradient gel electrophoresis(DGGE) of polymerase chain reaction-amplified(PCR) 16S rDNA fragments demonstrated that β-protebacteria, Actinomyces sp. and γ-protebacteria only exited in 1# reactor. The microbiological diversity of 1# reactor exceeded the other two reactors. Flavobacterium, Bacillales, Actinomyces, Actinobacteridae and uncultured bacteria(AF527584, AF502204, AY592749, AB076862, AJ619051, AF495454 and AY133070) could be detected in the biological phosphorus removal reactors. To investigate the microbial communities of microorganisms cultivated under different carbon sources, three sequencing batch reactors were operated. They were supplied with sewage, glucose and sodium acetate as carbon sources respectively and showed high phosphorus removal performance. The results of denaturing gradient gel electrophoresis(DGGE) of polymerase chain reaction-amplified(PCR) 16S rDNA fragments demonstrated that β-protebacteria, Actinomyces sp. and γ-protebacteria only exited in 1# reactor. The microbiological diversity of 1# reactor exceeded the other two reactors. Flavobacterium, Bacillales, Actinomyces, Actinobacteridae and uncultured bacteria(AF527584, AF502204, AY592749, AB076862, AJ619051, AF495454 and AY133070) could be detected in the biological phosphorus removal reactors.
出处 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2005年第4期611-614,共4页 环境科学学报(英文版)
基金 TheTeachingandResearchAwardProgramforOutstandingYoungTeachersinHigherEducationInstitutionsofMinistryofEducationofChina
关键词 16S rDNA carbon source biological phosphorus removal PCR-DGGE 16S rDNA carbon source biological phosphorus removal PCR-DGGE
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  • 1Carucci A, Lindrea K, Majone M et al., 1999. Different mechanisms for the anaerobic storage of organic substrates and their effect on enhanced biological phosphorus removal[J]. War Sci Tech, 39(6): 21-28.
  • 2Che O J, Dae S L, Jong M P, 2003. Microbial communities in activated sludge performing enhanced biological phosphorus removal in a sequencing batch reactor[J].War Res, 37: 2195-2205.
  • 3Comeau Y, Hall K J, Hancock R E W et al., 1986. Biochemical model for enhanced bioloagical phosphorus removal[J].Wat Res,20:1511- 1521.
  • 4Daims H, Ramsing N B,Schleifer K H et al., 2001. Cultivation-independent semiautomatic determination of absolute bacterial cell numbers in environmental samples by fluorescence in situ hybridization[J]. Appl Envir Microbia, 67:5810-5818.
  • 5Daniel B O, James D, Ebru D et al., 1998. Bioaugmentation of sequencing batch reactors for biological phosphors removal: comparative rRNA sequence analysis and bybridization with oligonucleotide probes[J]. Wat Sci Tec, 37(4/5):469-473.
  • 6Dabert P, Sialve B, Delgenes J P et al., 2001. Characterisation of the microbial 16S rDNA diversity of an aerobic phosphorus-removal ecosystem and monitoring of its transition to nitrate respiration[J]. Appl Environ Technolo,55:500-509.
  • 7Eschenhagen M, Schuppler M, Roske I et al, 2003. Molecular characterization of the microbial community structure in two activated sludge systems for the advanced treatment of domestic effluents[J]. Wter Res, 37:3224-3232.
  • 8Fuhs G W, Chen M, 1975. Microbiological basis of phosphate removal in the activated sludge process for the treatment of wastewater[J]. Microb Ecol, 2:119-138.
  • 9Gregory R C, Philip H, Philip L B et al. , 2002. Identification of polyphosphage-accumulation organisms and design of 16S rRNA-directed probes for their detection and quantitation[J]. Appl Environ Microbiol, 66:1175-1182.
  • 10Johwan A, Tomotaka D, Tsuneda S et al., 2002. Characterization of denitrifying phosphate-accumulation organisms cultivated under different electron acceptor conditions using polymerase chain reaction-denaturing gradient gel electrophoresis assay[J]. Wat Res, 36: 403-412.

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