摘要
对生活在不同温度下的1+龄史氏鲟血清及各组织中溶菌酶水平进行了测定,并对各温度下细菌感染后的史氏鲟溶菌酶水平变化进行了比较.史氏鲟血清和组织中溶菌酶的含量基本上随着温度的上升而升高,但对于不同的组织,溶菌酶含量在每个温度梯度之间变化的规律有所差别.在最低温和最高温时,血清、粘液、肝、胃及后肠的溶菌酶含量有显著性差异,其余各组织的溶菌酶随温度变化无显著性差异;溶菌酶在各组织中分布不同,前肠的溶菌酶量在各温度下均占据优势,幽门垂、后肠及脾中溶菌酶的量也较高,而粘液和血清中溶菌酶的量较低;嗜水气单胞菌感染后的史氏鲟血清溶菌酶量呈升高趋势,但变化不显著;肝脏除在14~15℃无变化外,其余温度下的溶菌酶含量与健康对照组有显著性升高,胃、鳃及粘液溶菌酶随温度产生不同的变化,史氏鲟前肠、后肠及幽门垂溶菌酶含量均降低.
Lysozyme levels in serum and other tissues of 1 + year old Amur sturgeon (Acipenser schrencki Brandt) were determined. Lysozyme content tended to increase with temperature, but the increasing range varied in different tissues. Lysozyme contents in serum, mucus, liver, stomach and hindgut showed significant differences between the lowest and the highest temperature in the test, while those in the other tissues showed no significant differences. The distribution of lysozyme in serum and several tissues of Amur sturgeon was analyzed. The results showed that lysozyme content in foregut was the highest in the tissues assayed in every temperature in the test, while lysozyme in pyloric caecum, hindgut and spleen showed high level in the test and that in serum and mucus was lower in level compared with other tissues. Lysozyme content in serum increased insignificantly after Amur sturgeon infected by Ah compared with that in the normal fish. Lysozyme in liver showed significant increase between normal fish and fish infected except at 14~15 ℃. Difference of lysozyme in stomach, mucus and gill between normal fish and infected fish varied in different temperature. Lysozyme in foregut, hindgut and pyloric caecum decreased after the fish infected by Ah. The changes of different tissues were not the same after the fish infected by Ah and the changes were related with the temperature.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2005年第3期63-66,共4页
Journal of Northeast Forestry University
关键词
史氏鲟
温度和感染
不同组织
溶菌酶含量
Amur sturgeon
Temperature and infection
Different tissues
Lysozyme level