单核细胞相关性白血病FCM免疫表型分析

Analysis of immunophonotype of 35 patients with monocyte lineage involved leukemia by flow cytometry

目的探讨单核细胞相关性白血病(MLIL)免疫表型与临床分型之间的关系及其在白血病的诊断、鉴别诊断、预后中的意义。方法采用CD45/SSC参数设门多色流式细胞术分析35例MLIL主要白血病相关抗原的表达情况。结果35例MLIL中,HLA-DR、CD117阳性率为89%和71%,CD14表达为57%,CD64表达为60%。在M5a中CD14未表达,在M5b中CD14表达为100%。在M5a中CD14、CD64与CD117表达呈负相关,而在M5b中,则呈正相关,部分MLIL病例可有CD+7,该部分病例完全缓解率仅为31.2%,预后不佳。结论MLIL肿瘤细胞起源早,特异性单核细胞分化抗原CD14、CD64敏感性较低,其结合CD117等可用于AML-M5的进一步分型,CD+7MLIL交叉表达患者完全缓解率低。

Objective To explore the correlation between the immunophenotype expression and the clinical features of 35 patients with monocyte lineage involved leukemia(MLIL) and to investigate its significance in diagnosis,differential diagnosis and the prognostic value for MLIL. Methods Bone marrow samples from 35 MLIL cases were analyzed by CD45/SSC double parameters and multicolor flow cytometry to determine the main leukocyte differentiation antigens. Results In 35 cases, the HLA- DR positive rate was 89 %, the positive rate of CD117?CD14?CD64 was 71 %?5 %?60 %, respectively. None of the 6 cases of M5a reacted the standard CD14 McAd, but the CD14 Positive rate in M5b was 100 %. In 6 cases of M5a, a significantly negative regression was found between CD14?CD64 and CD117. Otherwise, it was found positive regression in AML with M5b. A few MLIL cases express CD7 positively, these cases, complete recovery rate(CR) was only 31.2 %, it predicts a poor clinical outcome. Conclusions In the MLIL, CD14?CD64 expression rate was lower, accomplied with CD117 expression may be of help in distinguishing M5a and M5b. Lower CR rate was seen in the patients with CD7 positively expression than those with CD7 negatively expression.