以二氧化硅快速提取HBV－DNA用于双阶变温PCR检测

INTRAVENTRICULAR HEMORRHAGE TREATED BY VENTRICULAR DRILLING-DRAINAGE(REPORT OF 35 CASES)

以异硫氰酸胍裂解血清释放核酸使被二氧化硅吸附，经乙醇洗涤后用水悬浮，上清即含纯化ＤＮＡ。结合双阶变温ＰＣＲ建立一种简单快速的血清ＨＢＶ－ＤＮＡ检测方法。该法只需检测１０ｕｌ血清，完成提取和扩增只要３ｈ．操作很易掌握。用之检测５６例ＨＢｓＡｇ＋／ＨＢｅＡｇ＋的血清、４２例ＨＢｓＡｇ＋／ＨＢｅＡｇ－的血清和５４例ＨＢｓＡｇ／ＨＢｅＡｇ的血清，结果ＨＢＶ－ＤＮＡ检出率分别为８６％、５７％和１３％。应用表明这是一种简捷有效的核酸纯化和扩增方法，适于对大量临床样本作检测之用。

AbstractFrom 1985 to 1992, the authors have accumulated 35 cases of intraventricular hemor- rhage. Six cases were it simple intraventricular hemorrhage, and 29 cases were of cerebral hemorrhage ruptured into the ventricles. Twenty-two cases were unilateral or bilateral. The remaining 13 cases were intraventricular hemorrhage involving the two lateral ventricles and the 3rd ventricle , or the 3rd and the 4th ventricles. The causes of hemorrhage were hyper- tension (25 cases) , cerebral arteriovenous malformation (4 cases ) , and aneurysm ( 1 cases ) , and the remaining five were unknown. Depending upon the location and volume of hemor- rhage , the authors performed lateral or bilateral ventricular drilling-drainage for 2 to 25 days ( 60-380ml/day). Twenty-two cases survived and among them 8 cases completely recovered and returned to work as usual;13 cases died after therapy and 9 cases were complicated by pneumonia, gastrointestinal hemorrhage or intracranial infection.