纳米1,3,5-三氨基-2,4,6-三硝基苯的急性毒性和致突变性

Studies on acute toxicity and mutagenicity of nanometer TATB

目的了解纳米-1,3,5-三氨基-2,4,6-三硝基苯(1,3,5-triamino2,4,6-trinitrobenzene,nanoTATB)的急性毒性及致突变作用。方法对大、小鼠进行nanoTATB急性毒性实验和大鼠30d喂养实验。致突变选用Ames实验、小鼠骨髓嗜多染红细胞微核实验和小鼠精子畸形实验。结果急性毒性实验,2周内未见动物死亡,大、小鼠的经口急性毒性LD50均大于10g/kg,属实际无毒级;大鼠30d喂养实验中动物体重增加量、食物利用率、脏器系数及各项血液和生化指标与对照组比较,差异均无显著性(P>0.05);脏器组织病理学检查未见异常,估计nano-TATB经口染毒的最大无作用剂量大于6.0g/kg。3种致突变实验结果均为阴性。结论nano-TATB属实际无毒级化学物,无致突变作用。

Objective To determine the toxicity and mutagenicity effect of nanometer TATB(1,3,5-triamino-2,4,6-trinitrobenzene).Method Acute toxicity test,30 day feeding test in rats were used.Its mutagenicity was studied with Ames test,micronucleus test and sperm shape abnormality test.Result LD_(50) for nanometer TATB by oral exposure were above 10 g/kg in rats and mice.30 day feeding test showed that there were no significant differences in increased weight,food efficiency,relative organ weight and laboratory examination at the dose of 0.67,2.0 and 6.0 g/kg between animals exposed to nanometer TATB and the control group.Furthermore,no significant pathological changes were observed in liver,kidney,stomach,intestines,spleen,testicle and ovary in all animals.The maximal no-effect dose of nanometer TATB for oral exposure was above 6.0 g/kg.In the mutagenicity,no increase in the number of revertant colonies was found in the Ames tests with or without S9 mix.The micronucleus rates and sperm shape abnormality rates in all doses were no significant difference between the exposed groups and the control group(P>0.05),respectively.Conclusion Nanometer TATB is no actual toxicity and has no mutagenic effect in this study.