韩国淋巴瘤与中国延边地区淋巴瘤和鼻咽癌中EB病毒类型和30bp缺失型潜伏膜蛋白-1基因表达的比较研究

Comparison of EBV typing and 30 bp deleted LMP-1 gene in lymphoma of Korea and lymphoma and nasopharyngeal carcinoma of Yanbian of China

目的比较韩国淋巴瘤与中国延边地区淋巴瘤和鼻咽癌中EB病毒类型的分布特点。方法采用聚合酶链反应(PCR),巢式PCR,限制性酶切分析和Southern印迹杂交技术检测EB病毒的类型分布。结果在韩国非霍奇金淋巴瘤中,57%的B细胞淋巴瘤和75%的T细胞淋巴瘤表现为EBNA-1阳性。“C”变异型为44%,“f”变异型为9%。而在15例霍奇金淋巴瘤中,4例为“CF”变异型,11例为“DF”型。在延边地区,22%的淋巴瘤和81%的鼻咽癌表现为EBNA-1阳性。除1例“f”变异型以外,均为“F”型,“C”变异型占鼻咽癌的63%。此外,30bp缺失型潜伏膜蛋白-1基因在韩国淋巴瘤病例中占80%,未缺失型占14%,两者兼有者占6%。而在延边地区,缺失型在淋巴瘤中仅占43%,在鼻咽癌中占40%。结论EB病毒-1型为韩国和延边地区的主要EB病毒株。BamHI“f”变异型在两地区均罕见。但在韩国淋巴瘤病例中,30bp缺失型LMP-1基因的检出率则明显高于我国延边地区。

Objective To compare the distribution of different Epstein- Barr virus (EBV) types in Korea and Yanbian of China. Methods Polymerase chain reaction (PCR), nested- PCR and restriction enzyme cutting technology were used for EBV screening and typing, and Southern blotting was used for the detection of 30 bp deleted LMP- 1 gene. Results In Korean, about non- Hodgkin's lymphomas, 57 % of B cell lymphomas (BLs) and 75 % (15/20) of T cell lymphomas (TLs) were positive for EBNA- 1. C/D and F/f variant study classified the viruses as 'C' in 44 % and ' f ' in 9 % in malignant lymphomas (MLs), and 'CF' in 27 % and 'DF' in 73 % in Hodgkin's disease. In the cases from Yanbian of China, 22% of MLs and 81% nasopharyngeal carcinomas (NPCs) were positive for EBNA- 1. All cases were 'F' except one 'f' variant case. 'C' variant was 63 % in NPCs. In addition, 30 bp deleted LMP- 1 gene was 80 %, non- deleted form was 14 %, and both forms existed in 6 % of MLs in Korea. However, only 43 % of MLs and 40 % of NPCs were the deleted form in Yanbian of China. Conclusions EBV type 1 was ubiquitous strain in Korea and Yanbian of China. And BamHI ' f ' variant was rare in both areas. However, 30 bp deleted LMP- 1 gene was more common in Korea than in Yanbian of China.