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急性早幼粒细胞白血病患者胸腺近期输出功能情况 被引量：2

The thymic recent output function in patients with acute promyelocytic leukemia
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摘要 目的了解急性早幼粒细胞白血病(APL)患者T细胞受体重排删除DNA环(TRECs)的含量,从而了解患者胸腺近期输出na觙veT细胞的水平。方法利用实时定量PCR(TaqMan)方法检测9例APL患者和14例正常人外周血单个核细胞(PBMC)TRECs的水平,并根据外周血中CD3阳性率计算CD3细胞中TRECs水平。结果正常人TRECs拷贝数为(4.10±3.65)/1000PBMC和(6.36±5.28)/1000CD+3细胞,APL患者TRECs拷贝数为(0.13±0.22)/1000PBMC和(0.77±1.60)/1000CD+3细胞,与正常人相比均有显著性差异(P=0.0004,P=0.0005)。结论率先报道了APL患者胸腺近期输出na觙veT细胞功能明显降低的情况。 Objective To analyze the T- cell receptor excision DNA circles (TRECs) level and evaluate the thymic recent output nave T cells function in patients with acute promyelocytic leukemia (APL). Methods Quantitative detection of TRECs in DNA of peripheral blood mononuclear cells (PBMC) from 9 cases with APL were preformed by real- time PCR (TaqMan) analysis. The TRECs- number was related to the number of T- cells by determination of the number of CD-3 positive cells. 14 normal individuals served as controls. Results In comparison with normal individuals [ (4.10±3.65) copies/1000 PBMC and (6.36±5.28) copies/1000 CD+3 cells], a dramatic reduction of TRECs values in patients with APL [ (0.13±0.22) copies/1000 PBMC and (0.77±1.60) copies/1000 CD+3 cells] could be found (P =0.0004, P =0.0005). Conclusions This is, to our knowledge, the first description of TRECs level which was markedly reduced in APL patients.
作者 陈少华 李扬秋 杨力建 耿素霞
机构地区 暨南大学医学院血液病研究所
出处 《肿瘤研究与临床》 CAS 2005年第3期151-152,共2页 Cancer Research and Clinic
基金 国家自然科学基金(30270579) 广东省自然科学基金重点项目(23001) 广东省教育厅"千百十工程"优秀人才培养基金项目(Q校02022)
关键词 TRECS 胸腺近期输出功能 白血病 实时定量PCR TRECs Thymic recent output function Leukemia Real- time PCR
分类号 R733.71 [医药卫生—肿瘤]
  • 相关文献

参考文献6

  • 1Douek D C, McFarland R D, Keiser P H, et al. Changes in thymic function with age and during the treatment of HIV infection [J]. Nature, 1998, 396(6712):690-695.
  • 2McFarland R D, Douek D C, Koup R A, et al. Identification of a human recent thymic emigrant phenotype[J]. PNAS, 2000, 97(8):4215-4220.
  • 3李扬秋,韩素芳,杨力建.胸腺输出功能的评价手段—T细胞受体重排删除环的定量分析[J].中国实验血液学杂志,2003,11(6):667-672. 被引量：24
  • 4耿素霞,李扬秋,杨力建,陈少华,韩素芳,周羽竝,陈青山.实时定量PCR分析CML患者胸腺近期输出功能[J].肿瘤防治研究,2004,31(6):317-319. 被引量：10
  • 5Chen X, Barfield R, Benaim E, et al. Prediction ofT-cell reconstitution by assessment of T-cell receptor excision circle before allogeneic hematopoietic stem cell transplantation in pediatric patients[J]. Blood,2005, 105(2):886-893.
  • 6Petridou E, Klimentopoulou A E, Moustaki M, et al. Recent thymic emigrants and prognosis in T- and B- cell childhood hematopoietic malignancies[J]. Int J Cancer, 2002, 101(1):74-77.

二级参考文献30

  • 1[1]Hazenberg MD, Verschuren MC, Hamann D, et al. T cell receptor excision circles as markers for recent thymic emigrants: basic aspects, technical approach, and guidelines for interpretation. J Mol Med, 2001; 79:631-640
  • 2[2]Ye P, Kirschner DE. Reevaluation of T cell receptor excision circles as a measure of human recent thymic emigrants. J Immunol, 2002;168:4968-4979
  • 3[3]Douek DC, McFarland RD, Keiser PH, et al. Changes in thymic function with age and during the treatment of HIV infection. Nature, 1998; 396(6712): 690-695
  • 4[4]Petridou E, Klimentopoulou AE, Moustaki M, et al. Recent thymic emigrants and prognosis in T- and B-cell childhood hematopoietic malignancies. Int J Cancer, 2002; 101: 74-77
  • 5[5]Koetz K, Bryl E, Spickschen K, et al T cell homeostasis in patients with rheumatoid arthritis. Proc Natl Acad Sci USA, 2000;97: 9203 - 9208
  • 6[6]Talvensaari K, Clave E, Douay C, et al. A broad T-cell repertoire diversity and an efficient thymic function indicate a favorable longterm immune reconstitution after cord blood stem cell transplantation. Blood, 2002; 99:1458-1464
  • 7[7]AL-Harthi L, Marchetti G, Steffens CM, et al. Detection of T cell receptor circles (TRECs) as biomarkers for de novo T cell synthesis using a quantitative polymersse chain reaction-enzyme linked immunosorbent assay (PCR-ELISA). J Immunol Medthods, 2000; 237:187 - 197
  • 8[9]Poulin JF, Viswanathan MN, Harris JM, et al. Direct evidence for thymic function in adult humans. J Exp Med, 1999; 190:479-486
  • 9[10]Kimmig S, Przybylski G, Schrnidt CA, et al. Two subsets of naive T helper cells with distinct T cell receptor excision circle content in human adult peripheral blood. J Exp Med, 2002; 195:789-794
  • 10[11]Markert ML, Boeck A, Hale LP, et al. Transplantation of thymus tissue in complete DiGeorge syndrome. N Engl J Med; 1999; 341:1180-1189

共引文献30

同被引文献17

  • 1李扬秋,杨力建,陈少华,周羽竝,耿素霞秋,陈青山.AML-M5患者外周血naiveT细胞水平和TCRVβ谱系利用特点[J].现代临床医学生物工程学杂志,2004,10(4):283-286. 被引量：6
  • 2李扬秋,韩素芳,杨力建,陈少华,周羽竝,陈嘉愉,刘薇薇.重度苯中毒患者胸腺输出近期功能明显低下[J].中国实验血液学杂志,2005,13(1):114-117. 被引量：18
  • 3Douek DC,McFarland RD,Keiser PH,et al.Changes in thymic function with age and during the treatment of HIV infection.Nature,1998,396:690-695.
  • 4Bertho JM,Demarquay C,Moulian N,et al.Phenotypic and immu-nohistological analyses of the human adult thymus:evidence for an active thymus during adult life.Cell Immunol,1997,179:30-40.
  • 5McFarland RD,Douek DC,Koup RA,et al.Identification of a human recent thymic emigrant phenotype.PNAS,2000,97:4215-4220.
  • 6Li YQ.Recent thymic output function in patients with hematological malignancy.Hematology,2005,10:297-305.
  • 7Jimenez M,Martinez C,Ercilla G,et al.Clinical factors influen-cing T-cell receptor excision circle (TRECs) counts following allogeneic stem cell transplantation in adults.Transli Immunol,2006,16:52-59.
  • 8Petridou E,Klimentopoulou AE,Moustaki M,et al.Recent thymic emigrants and prognosis in T-and B-cell childhood hematopoietic malignancies.Int J Cancer,2002,101:74-77.
  • 9Torelli GF, Paolini R, Tatarelli C, et al. Defective expression of the T-cell receptor-CD3 zeta chain in T-cell acute lymphoblastic leukaemia [J]. Br J Haematol, 2003,120 (2) :201-208.
  • 10Atkinson TP, Hall CG. Splice variant in TCR-ZETA links T cell receptor signaling to a G-protein-related signaling pathway[J]. Biochem Biophysi Res Commun, 2003,310(2003) :761-766.

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肿瘤研究与临床
2005年 第3期

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