松毛虫赤眼蜂工厂化产品低温贮存的研究

Studies on Low Temperature Storage in Industrial Production of Trichogramma dendrolimi Matsumura

在3℃恒温条件下,对非滞育的松毛虫赤眼蜂的小幼虫期、幼虫中期、老熟幼虫期、预蛹期、蛹初期和蛹中期6个虫态进行了长期的贮存试验。结果表明,不同贮存虫态对其贮存效果有较大影响,幼虫中期虫态贮存效果最佳,小幼虫期次之。小幼虫期和幼虫中期虫态贮存期不宜超过60d,其他虫态不宜超过15d。贮存期在20d内,单卵蜂数随贮存期的延长而下降,20d之后单卵蜂数变化基本稳定,小幼虫期贮存单卵蜂数下降较多。贮存期长短与成蜂单雌产卵数的变化关系不明显,但蛹初期和蛹中期虫态贮存成蜂的单雌产卵数和可育蜂数量明显下降。

A long-term storage test under constant temperature of 3℃ was carried out to undiapause Trichogramma dendrolimi of six stages of growth including young larvae, middle larvae, old larvae, prepupa, initial pupa, middle pupa. The results showed that the storage effect was influenced by different stage of growth. The middle larva is the best stage and young larva is better stage for storage. The storage time of young larvae and middle larvae may not exceed sixty day, others may not exceed fifteen days. As the storing lasted, the number of Trichogramma dendrolimi in a single host egg reduced in first twenty days. Later, it no longer changed. If young larva was stored, the number of Trichogramma dendrolimi in a single host egg reduced greatly. The change of the single female adult oviposition was not significantly correlated with the length of storage. In case initial pupa and middle pupa were stored, the number of the single female adult oviposition reduced greatly.