肠系膜淋巴管结扎对MODS大鼠体液免疫功能的影响

Effect of Mesenteric Lymph Duct Ligation on Humoral Immunity Function in MODS Rats

目的观察结扎肠系膜淋巴管对二次打击致大鼠多器官功能障碍综合征(MODS)体液免疫功能的影响,探讨淋巴途径在MODS发病学中的意义。方法雄性Wistar大鼠均分为结扎组、未结扎组、假手术组三组。前两组以失血-LPS二次打击方法,复制大鼠MODS模型。所有动物实验前后的血清均以免疫比浊法检测IgG、IgA、IgM、C3、C4含量,同时以放射免疫法检测血清TNFα作为反映炎症的指标。结果成功复制了MODS大鼠模型。二次打击后,未结扎组大鼠血清IgG、IgA、IgM、C3、C4及TNFα均显著高于结扎组、假手术组及实验前(P<0.01～0.05),而结扎组与假手术组无显著差异(P>0.05)。结论失血-LPS二次打击可使大鼠免疫球蛋白及补体增多,反映炎症反应剧烈及抗炎反应增强,而肠系膜淋巴管结扎可缓解炎症与抗炎反应,提高存活率,对机体有较好的保护作用。

Objective To study the effects of mesenteric lymph duct ligation on humoral immunity function in multiple organ dysfunction syndrome (MODS) rats subjected to two-hit, and observe the meaning of lymph pathway in terms of MODS. Methods Male wistar rats were divided into three groups: the mesenteric lymph duct ligation group, the non-ligation group and sham group. The MODS model was established using two-hit mothod of hemorrhage and LPS in ligation group and no-ligation group. The immunoglobulin G (IgG), IgA, IgM and complement 3 (C_3), C_4 in serum were determined by immunoprecipitation method before and after experiment, and the turnor necrosis factor-alpha (TNFα) was determined by radioimmunoassay reflecting inflammation. Results It showed that the MODS model was established successfully. After two-hit, the IgG, IgA, IgM, C_3, C_4 and TNFα in serum of non-ligation group were obviously increased than those of ligation group, sham group and before experiment (P<0.01～0.05), and there was no significant difference in these indexes between ligation group and sham group (P<0.05). Conclusion Immunoglobulin and complement increased in MODS rats subjected to two-hit of hemorrhage/ resuscitation and LPS, it suggested that inflammatory and anti-inflammatory response was enhanced, and the ligation of mesenteric lymph duct might relax the inflammatory and anti-inflammatory response, raise the survival rate,which had a fairly protective effect.