摘要
参照国外发表的ＣＤ２３全基因序列，自行设计并合成一对ＤＮＡ引物（２５个碱基和１８个碱基），ＵＣＤ２３阳性细胞株ＨＦＢｄ２／ＳＤＮＡ为模板，应用ＰＣＲ技术，在国内首次成功地扩增了ＣＤ２３全基因（９８６ｂｐ），为今后克隆和表达ＣＤ２３基因以及进一步在基因水平上探讨ＣＤ２３的生物学功能研究中提供了物质基础。
Abstract With reference to the published sequence of the human CD23 gene , a pair of DNA primers (25 bases and 18 bases ) were designed and synthesized. A 986 base pair fragment containing full CD23. cDNA gene was successfully amplified by using the polymerase chain reaction (PCR ). It was the first report in the country and would promote the further re- search in molecular biology of CD23.
出处
《中山医科大学学报》
CSCD
1994年第4期246-248,共3页
Academic Journal of Sun Yat-sen University of Medical Sciences
基金
国家自然科学基金
卫生部青年科学基金
