定量蛋白质组同位素标记技术及应用

Quantitative Proteomics Using Isotope Labeling Techniques and Its Recent Application

定量蛋白质组学是对蛋白质组进行精确的定量和鉴定的学科,突破了传统蛋白质组研究集中于对蛋白质的分离和鉴定,着重于定性定量解析细胞蛋白质的动态变化信息,更真实地反映了细胞功能、过程机制等综合信息。以同位素为内标的质谱分析新技术的提出,显示出可同时自动鉴定和精确定量的能力,代表了目前定量蛋白质组研究的主要发展方向。对近年来定量蛋白质组学同位素标记技术和应用研究所取得的重要进展以及最新的发展动态进行了综述。

Quantitative proteomics is defined as the systematic identification and the determination of quantitative change in the complete proteome. Quantitative proteomics focus on the information of dynamical changes of proteins of complex samples, and is expected to provide new functional insights into the processes of biological systems. The use of stable isotopes as internal standards in mass spectrometry has opened a new era for quantitative proteomics and has the potential for studying on proteins by identifying and quantifying automatically and simultaneously. Advance in isotope labeling techniques and recent biological applications are reviewed, further de- velopment based on quantitative proteomics is discussed.