乙酸铜对蚕豆根尖细胞致畸效应

Teratogenic Effect of Copper Acetic Acid on Vicia faba Root Tip Cells

采用蚕豆根尖细胞的微核试验和染色体畸变试验方法,以不同浓度的乙酸铜为诱变剂,选择不同的处理时间,测定蚕豆根尖细胞的有丝分裂指数、微核率和染色体畸变率。结果表明:乙酸铜能诱发较高频率的微核率,处理6h、12h时微核率均随着乙酸铜浓度的升高而增加,具有明显的剂量效应;处理24h时在实验浓度范围内,其微核率随乙酸铜浓度的升高而增加,但高于一定浓度后反而呈下降趋势。不同浓度的乙酸铜在不同处理时间均使蚕豆根尖细胞有丝分裂指数增大。乙酸铜还能诱导蚕豆根尖细胞产生较高频率的染色体畸变,且产生多种类型的染色体畸变。因此,乙酸铜对蚕豆根尖细胞具有明显的致畸效应。

With different concentrations of copper acetic acid as mutagen, we used the micronucleus assay and chromosome aberration assay to determine mitotic index, micronucleus rate and chromosome aberration rate of Vicia faba root tip cells. The result showed that copper acetic acid could increase the micronucleus rate of Vicia faba root tip cells. Within certain range of concentration of copper acetic acid and treating for 6 h and 12 h, the rate of micronucleus was found to increase systematically with the added concentration of copper acetic acid. Within certain range of concentration of copper acetic acid and treating for 24 h, the rate of micronucleus was found to increase systematically with the added concentration of copper acetic acid, and then to decrease systematically with the concentration. At different time of treatment, different concentrations of copper acetic acid could apparently increase mitotic index. Copper acetic acid also caused high rate and various types of chromosome aberration. Thus, copper acetic acid had systematical teratogenic effects on Vicia faba root tip cells.