沙眼衣原体感染后大鼠输卵管与植物凝集素BS-1结合的糖蛋白的变化

CHANGES IN RAT OVIDUCTAL GLYCOPROTEIN COMBINED WITH BS-1 AFTER CHLAMYDIAL TRACHOMATIS INFECTION

目的研究沙眼衣原体感染后输卵管与植物凝集素BS-1结合的糖蛋白的变化。方法取25只成熟Wistar雌性大鼠随机分为实验组和对照组,每组5只。对照组接种2SP代替沙眼衣原体,其余每组大鼠均在卵巢囊接种沙眼衣原体D型株。分别于感染后3,5,7,14d处死动物,取输卵管,观察大鼠输卵管与植物凝集素BS1结合的糖蛋白在感染后不同天数发生的改变,其变化可通过荧光显微镜观察与植物凝集素BS1结合后的荧光强度来检测,并采用HPIAS1000图文分析系统进行定量分析和SPSS11.0统计软件作统计分析。结果输卵管受损部位主要在粘膜层,富含N乙酰D半乳糖胺的糖蛋白随感染的天数不同,其变化有所不同,尤其以输卵管峡部变化明显。图像分析结果显示:感染第7d时其分泌量最低,感染后第14d其分泌量逐渐恢复,且各组间差异有显著性意义(P<0.05)。结论输卵管是精子与卵子结合的地方,也是胚胎早期发育的场所。富含N乙酰D半乳糖胺的糖蛋白可通过参与精卵结合、受精卵卵裂和早期胚胎发育等方式,对受精卵及早期胚胎发挥保护作用。

Objective To study the alteration in the glycoprotein combined with BS-1 on the mucosal surface of the oviduct after chlamydial trachomatis (CT) infection. Methods 25 mature femal Wistar rats were randomly divided into 4 experimental and 1 control groups, with 5 rats each. The rats of the experimental groups were inoculated with CT(immunotype D)into two sides of the ovarian bursa,while those of the control group with 2SP instead. The rats were killed on the day 3, 5, 7, 14 after the inoculation, respectively. The changes in the glycoprotein combined with BS-1 were observed under fluorescence microscope,and a quantitative analysis was conducted with HPIAS-1000 image pattern analysis system. Results The lesions were limited to the oviductal mucosa. Fluodensitometry results showed that the glycoprotein rich in N-acetyl-D-galactosamine varied with infection time, especiallyin the isthmus of the oviduct. Image pattern analysis results showed that the amount of ghcoprotein secrection wasthe lowest on the day 7 and slowly recovered on the day 14 and there were statistical differences between 4 groups in the amount of its secrection(P<0.05). Conclusion The oviduct is the site of sperm-egg interaction and blastomere embryo development. The glycoproteins rich in N-acetyl-D-galactosamine may play an important role for protecting zygoteand early embryo against attacks from their environment.