神经生长因子促进坐骨神经再生修复的酶组织化学研究

ENZYMOHISTOCHEMICAL STUDY OF THE PROMOTING EFFECT OF NERVE GROWTH FACTOR ON INJURED SCIATIC NERVE

目的研究对兔右坐骨神经损伤后局部给予蛇毒神经生长因子(NGF),观察坐骨神经酶活性变化和超微结构的恢复情况,探讨NGF对神经再生的影响。方法乙酰胆碱酯酶(AChE)、酸性磷酸酶(ACPase)的酶组织化学技术和电镜技术。结果神经损伤后:AChE活性明显下降,NGF组的AChE活性恢复快于盐水对照组;ACPase活性逐渐增高,NGF组的ACPase活性恢复时间短于盐水对照组。坐骨神经的超微结构在神经损伤后也发生变化,NGF组的变化程度小于盐水对照组,恢复时间短于对照组。结论NGF可通过影响酶物质的代谢而起到加快受损神经恢复的作用。为临床上应用蛇毒NGF治疗周围神经损伤提供形态学依据。

Objective Rabbits with crushed right sciatic nerves were locally sprayed with snake venom nerve growth factor (NGF). The enzyme activity and the ultrastructure of sciatic nerve were observed, and the effect of NGF on the regeneration of injured nerve was explored. Methods Acetylcholinesterase(AChE) and acid phosphatase (ACPase) changes were observed with enzymohistochemistry and electron microscopy. Results AChE activity obviously decreased after crush, and recovered faster in NGF group than that in control group sprayed with physiological saline. Meanwhile, ACPase activity gradually increased after crush and the recovery interval in NGF group was shorter than that in control group. After injury, the changes of ultrastructure of sciatic nerve could also be seen. It was less change in NGF group than that in control group and recovered faster in NGF group than that in control group. Conclusion NGF may accelerate the recovery of injured nerve by effecting enzymatic metabolism. This study can provide morphological proof for clinical use of snake venom NGF to treat peripheral nerve injury.