2,4-D浓度对花生体细胞胚发生的影响

Effect of 2,4-D Concentration on Somatic Embryogenesis in Peanut

选用丰花2号、鲁花11和农大818三个花生品种的胚小叶为外植体,在MSB培养基中分别添加不同浓度的2,4-D(1、5、10、15、20mgL)作为诱导培养基,以MSB为继代培养基,研究2,4-D浓度对花生体细胞胚胎发生的影响。结果表明:2,4-D浓度对花生胚小叶脱分化及再分化有显著影响,低浓度的2,4-D对外植体脱分化有利,而较高浓度对再分化有利,诱导体细胞胚的最适2,4-D浓度为15mgL。不同品种的体细胞胚诱导频率存在差异,丰花2号和农大818比鲁花11的体细胞胚诱导频率高;且丰花2号的成苗能力较强,在MSB培养基上即可成苗,鲁花11及农大818成苗能力较差。

Embryonic leaflets of 3 peanut varieties Fenghua 2, Luhua 11 and Nongda 818 were cultured on a modified MSB medium respective containing 1, 5, 10,15 or 20 mg/L 2,4-D, Studying the effect of 2,4-D concentration on somatic embryogenesis of peanut showed that de-differentiation and re-differentiation of explants were influenced by concentration of 2,4-D : Lower concentration of 2,4-D was propitious to de-differentiation and higher concentration was propitious to re-differentiation. The best concentration of 2,4-D was 15mg/L. Different varieties have different frequency of embryogenesie. Frequences of Fenghua2 and Nongda818 were higher than that of Luhua11. Seedlings easily form on MSB medium in Fenghua 2, and difficulty in Luhua 11 and Nongda 818.