期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

副溶血性弧菌污染情况调查

Investigation of Vibrio parahaemolylicus pollusion
下载PDF
导出
摘要 目的对广州市海珠区的海产品等食品及腹泻病人粪便标本共86份进行了副溶血性弧菌的检测,其中海产品51份,熟食5份,咸菜3份,腹泻病人粪便标本27份。方法按照《广东省卫生防疫病原微生物检验常规》和尿素酶试验进行检验。结果食品样品中分离到副溶血性弧菌5株,阳性率8.47%,按尤氏生化分型为I型2株,II、IV、V型各1株,病人粪便均未分离到此菌。结论尿素酶试验在致病学鉴定上具有重要意义。 Objective To investigation the Vibrio parahaemolylicus in marine products and stool spcimen. Methods According to the rule of aetiology examination of preventive medicine of Guangdong and urea enzyme test. Results 5 Vibrio parahaemolylicus has been isolated in marine products by positive rate 8.47%, while not in stool specimen. Conclusions The urea enzyme test is very significant and common method for Vibrio parahaemolylicus identification.
作者 刘爱红
机构地区 东莞市疾病预防控制中心
出处 《国际医药卫生导报》 2005年第11期106-108,共3页 International Medicine and Health Guidance News
关键词 副溶血性弧菌 污染情况 尿素酶试验 广州市海珠区 粪便标本 腹泻病人 微生物检验 卫生防疫 食品样品 生化分型 重要意义 海产品 广东省 阳性率 人粪便 致病学 分离 Vibrio parahaemolylicus marine products urea enzyme test
分类号 R155 [医药卫生—营养与食品卫生学]
  • 相关文献

参考文献9

  • 1陆德源.医学微生物学(第三版)[M].人民卫生出版社,1989.147.
  • 2田贵勤.一起副溶血性弧菌引起的食物中毒[J].广东卫生防疫,1995,21(2):76-77. 被引量：2
  • 3余亚红.副溶血性弧茵噬茵体的研究[J].国外医学流行病学传染病学分册,1991,18(2):71-72.
  • 4柳丽江.副溶血性弧茵引起的食物中毒报告[J].国外医学流行病学传染病学分册,1991,11(2):71-72.
  • 5.食品中毒-嗜盐茵感染专辑 上海市科学技术编译本[M].,1964..
  • 6蒋贤根.副溶血性弧茵食物中毒流行病学判定依据的探讨[J].国外医学微生物学分册,2000,6(1):12-12.
  • 7丁琦.一起副溶血性弧茵食物中毒的调查[J].中华预防医学杂志,1999,18(2):65-65.
  • 8卢鹤新.副溶血性弧茵引起食物中毒的分析[J].国外医学微生物学分册,2001,14(5):218-218.
  • 9谭翰清,万成松.副溶血性弧菌快速检测研究进展[J].华南预防医学,2004,30(1):21-23. 被引量：13

二级参考文献17

  • 1[1]Gooch JA,Depaolar A,Kaysmer CA et al.Evaluation of two direc plating methods using nonoradio active probes for enumeration of vibrio parahaemolyticus in oysters[J].Appl and Environ Microbiol,2001,67(2):721-724.
  • 2[2]Okuda J,Nishibuchi M.Manifestation of the kanagawa phenomenon,the virulence-associated phenotype,of vibrio parahaemolyticus depends on a particular single base change in the promoter of the thermostable direct haemolysingene[J].Mol Microbiol,1998,30(3): 499-511.
  • 3[3]Iguchi T,Kondo S,Hisatsune K.Vibrio parahaemolyticus oserotype from O1 to O13 all polymerase r-type:SDS-PAGE and compositional sugar analysis[J].FEMS Microbiol Lett,1995,130(2-3): 287-292.
  • 4[4]Myers ML,Panicker G,Bej AK.PCR detection of a newly emerged pandemic vibrio parahaemolyticus 03:06 pathogen in pure cultures and seeded waters from the gulf of mexico[J].Appl Environ Microbiol,2003 ,69(4): 2194-2200.
  • 5[5]Bej AK,Patterson DP,Brasher CW,et al.Detection of total and hemolysin-producing vibrio parahaemolyticus in shellfish using multiplex PCR amplification of tl,tdh and trh[J].J Microbiol Methods,1999,36 (3):215-225.
  • 6[6]Suthienkul O,Iida T,Park KS,et al.Restriction fragment length polymorph- ism of the tdh and trh genes in clinical vibrio parahaemolyticus strains[J].J Clin Microbiol,1996 ,34(5):1293-1295.
  • 7[7]Honda T,Miwatani T,Yabushita Y,et al.A novel method to chemically immobilize antibody on nylon and its application to the rapid and differential detection of two vibrio patahaemolyticus toxins in a.modified enzyme-linked immunosorbent assay[J].Clinical and diagnostic laboratory immunology,1995,2(2):177-181.
  • 8[8]Okuda J,Ishibashi M,Hayakawa E,et al.Emergence of a unique O3:K6 clone of vibrio parahaemolyticus in Calcutta.India,and isolation of strains from the same clonal group from Southeast Asian travelers arriving in Japan[J].J Clin Microbiol,1997,35(12):3150-3155.
  • 9[9]Yamamoto K,Honda T,Miwatani,et al.Enzyme-labeled oligonucleotide probes for detection of the genes for thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) of vibrio parahaemolyticus[J].Can J Microbiol,1992,38(5):410-416.
  • 10[10]Lee CY,Panicker G,Bej AK.Detection of pathogenic bacteria in shellfish using multiplex PCR followed by covalink NH microwell plate sandwich hybridezation[J].J Microbiol Methoeds,2003,53(2):199-209.

共引文献13

国际医药卫生导报
2005年 第11期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部