期刊文献+

人牙周韧带细胞和β-磷酸三钙多孔生物陶瓷的牙周组织工程研究 被引量:3

A Study of Tissue Engineered Periodontal Ligament Based on Human Periodontal Ligament Cells and β-TCP.
下载PDF
导出
摘要 目的:以多孔β-磷酸三钙(β-TCP)生物陶瓷材料作为组织工程支架,以人牙周韧带细胞(PDLCs)作为种子细胞,探索构建组织工程化牙周膜的可行性。方法:将体外培养7d的PDLCs和β-TCP的复合物,植入裸鼠背部一侧皮下,在对称部位植入单纯的β-TCP作为空白对照。术后4周、8周、12周分别取材,进行组织学检测。结果:实验组8周后支架材料部分吸收,可见未降解的支架材料孔洞内广泛分布着新生组织,其内可见小血管、腺体等结构;12周时支架材料大部分吸收,取而代之的是大量片状的新生结缔组织。空白组组织形成较少,支架材料降解与实验组相似。结论:体外培养的PDLCs与β-TCP复合后,能在体内增殖、分化,形成结缔组织,从而证明以β-TCP作为支架材料进行的牙周组织工程是可行的。 Objective: To investigate the possibility of generate tissue engineered periodontal ligament by using porous β-TCP as scaffold and human periodontal ligament(PDL) cells as seed cell. Methods: PDLCs were seeded onto scaffolds and implanted subcutaneously in BALB/c-nu mice. The control group were implanted by β-TCP only. Formed tissues were harvested at 4,8,12 weeks after operation for histological analysis. Results: In the experimental group, histological analysis demonstrated obvious degradation of β-TCP and extensive new formation at 8 weeks after operation. The degradation of β-TCP was even obvious at 12 weeks after operation, and the cells on β-TCP formed sparse monolayers as well as the connected tissues. In the control group, only a small quantity of new formation could be seen. Conclusion: It is feasible to generate tissue engineered periodontal ligament by using porous β-TCP as scaffold and hPDLCs as seed cell.
出处 《口腔医学研究》 CAS CSCD 2005年第3期243-245,共3页 Journal of Oral Science Research
基金 国家自然科学基金资助项目(编号:30171021)
关键词 组织工程化牙周膜 牙周韧带细胞 Β-磷酸三钙 Tissue engineering Human periodontal ligament cells β-tricalcium phosphate
  • 相关文献

参考文献3

二级参考文献11

共引文献38

同被引文献41

  • 1骆凯,闫福华,金岩,聂鑫,刘源,王新文.牛肌腱复合胶原与人牙周膜成纤维细胞培养的实验研究[J].中国修复重建外科杂志,2005,19(3):234-237. 被引量:10
  • 2许杰,吴织芬,储庆,董广英,魏红宇,刘玲侠.牙周组织工程中人转化生长因子基因转染人牙龈成纤维细胞的生物学特性[J].中国临床康复,2005,9(10):48-50. 被引量:1
  • 3田敏,王贻宁,陈新明,张翼.PGA无纺网与PDLCs的3D共培养物在裸鼠体内的生长观察[J].口腔医学研究,2005,21(2):116-118. 被引量:3
  • 4Bartold PM, Mcculloch CA, Narayanan AS, et al. Tissue engineering: a new paradigm for periodontal regeneration based on molecular and cell biology [ J ]. Periodontol 2000, 2000,24( 11 ) : 253-269.
  • 5lvanovski S, Gronthos S, Shi S, et al. Stem cells in the periodontal ligament [J]. Oral Diseases, 2006, 12(4) :358-363.
  • 6Byoung MS, Masako M, Stan G, et al. Investigation of muhipotent postnatal stem cells from human periodontal ligament [ J ]. Lancet, 2004, 364(9429) :149-155.
  • 7Bruckner P, Horler 1, Mendler M, et al. Induction and prevention of chondrocyte hypertrophy in culture [J]. J Cell Biol, 1989, 109(5): 2537-2545.
  • 8Aeil Y, Terheyden H, Dunsche A, et al. Three-dimensional cultivation of human osteoblast-like cells on highly porous natural bone mineral [J]. J Biomed Mater Res, 2000, 51(4):703-710.
  • 9Ragnarsson B, Carr G, Daniel JC. Isolation and growth of human periodontal ligament cells in vitro [J]. Dent Res, 1985, 64 (8) :1026- 1030.
  • 10Adams AM, Soames JV, Seral RF. Cultural and morphological characteristics of human periodontal ligament cells in vitro [ J ]. Arch Oral Biol, 1993, 38(8) :657-662.

引证文献3

二级引证文献8

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部