人牙周韧带细胞和β-磷酸三钙多孔生物陶瓷的牙周组织工程研究

A Study of Tissue Engineered Periodontal Ligament Based on Human Periodontal Ligament Cells and β-TCP.

目的:以多孔β-磷酸三钙(β-TCP)生物陶瓷材料作为组织工程支架,以人牙周韧带细胞(PDLCs)作为种子细胞,探索构建组织工程化牙周膜的可行性。方法:将体外培养7d的PDLCs和β-TCP的复合物,植入裸鼠背部一侧皮下,在对称部位植入单纯的β-TCP作为空白对照。术后4周、8周、12周分别取材,进行组织学检测。结果:实验组8周后支架材料部分吸收,可见未降解的支架材料孔洞内广泛分布着新生组织,其内可见小血管、腺体等结构;12周时支架材料大部分吸收,取而代之的是大量片状的新生结缔组织。空白组组织形成较少,支架材料降解与实验组相似。结论:体外培养的PDLCs与β-TCP复合后,能在体内增殖、分化,形成结缔组织,从而证明以β-TCP作为支架材料进行的牙周组织工程是可行的。

Objective: To investigate the possibility of generate tissue engineered periodontal ligament by using porous β-TCP as scaffold and human periodontal ligament(PDL) cells as seed cell. Methods: PDLCs were seeded onto scaffolds and implanted subcutaneously in BALB/c-nu mice. The control group were implanted by β-TCP only. Formed tissues were harvested at 4,8,12 weeks after operation for histological analysis. Results: In the experimental group, histological analysis demonstrated obvious degradation of β-TCP and extensive new formation at 8 weeks after operation. The degradation of β-TCP was even obvious at 12 weeks after operation, and the cells on β-TCP formed sparse monolayers as well as the connected tissues. In the control group, only a small quantity of new formation could be seen. Conclusion: It is feasible to generate tissue engineered periodontal ligament by using porous β-TCP as scaffold and hPDLCs as seed cell.