老年男性维生素D受体基因多态性与骨密度关系的研究

Study on the relationship between vitamin D receptor gene polymorphisms and bone mineral density in old man

目的研究维生素D受体(vitamin D receptor,VDR)基因多态性在老年男性中的分布, 并进一步研究其与骨密度的关系。方法采用聚合酶链反应-限制性片段长度多态性(PCR- RFLP)方法,分析145例老年男性的VDR基因型,同时用双能X线吸收法测定腰椎及髋部骨密度。结果 VDR基因型分别为BB,0．014;Bb,0．117;bb,0．869。骨质疏松组与非骨质疏松组之间VDR基因型分布频率的差异无显著性(P>0．05)。比较各基因型组的骨密度,bb组及 Bb组只有在股骨颈处显示出BMD均低于BB组,差异有显著性(P<0．05),其它部位,三个基因型组的BMD均差异无显著性(P>0．05)。结论老年男性VDR基因型分布频率与某些西方国家人群分布不同,其VDR基因型与骨密度无明显相关性。VDR基因可能不是我们所研究群体 BMD的主要遗传基因。

Objective To investigate the frequency distribution of vitamin D receptor (VDR) genotype in Chinese old men, and evaluate its relationship with VDR gene polymorphisms and bone mineral density (BMD) .Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFCP) were used to determine VDR genotypes in 145 old men. BMD of the lumbar spines and hips were measured by dual-energy X-ray absorptiometry (DEXA) . Results The frequency distribution of the VDR genotypes BB, Bb, bb was 0. 014, 0. 117, 0. 869 respectively. No significant difference was found in genotypes distribution between the groups of osteoporosis and non-osteoporosis. BMD of 'bb' or 'Bb' genotype was lower than 'BB' genotype only in femoral neck. Conclusions The frequency distribution of the VDR genotype in Chinese old men is different from that in Western countries. VDR genotype has no correlation with BMD and bone metabolic markers and may not be the inherit gene of BMD in our study objects.