摘要
目的研究重组变构人TRAIL(rmhTRAIL)对人非小细胞肺癌(NSCLC)细胞和人正常细胞的生长、抑制作用及其与化疗药的联合作用。方法采用四唑盐(MTT)和集落形成试验法检测细胞的生长,并观察了与顺铂(DDP)联合应用对细胞生长的作用。结果rmhTRAIL对多种NSCLC细胞有显著的抑制作用,而对人正常细胞无影响;rmhTRAIL与DDP联合应用具有协同或增强对NSCLC细胞的抑制作用,而且对正常人细胞不表现毒性。结论rmhTRAIL是一种安全、有效的抗肿瘤生物制品。
Objectives To investigate the inhibitory effects of recombinant mutant human TRAIL (rmhTRAIL) on human NSCLC cells and normal cells and the combined efficacy with chemo-therapy medicine. Methods The growth of cells was examined with MTT assay and colony forming assays. We also observed the synergy of rmhTRAIL and cisplatin (DDP) on growth of cells. Results rmhTRAIL has significant inhibitory effects on several NSCLC cells, but not on human normal cells. Synergic or enhanced inhibitory effects on NSCLC cells were also observed when rmhTRAIL was used together with DDP and there was no toxicity on human normal cells. Conclusion rmhTRAIL is a safe and effective anti-tumor bio-product.
出处
《基础医学与临床》
CSCD
北大核心
2005年第6期512-516,共5页
Basic and Clinical Medicine
基金
科技型中小企业技术创新基金(04C26211100286)
