摘要
目的:建立以高效液相色谱法测定蝎子七中没食子酸含量的方法。方法:色谱柱为Nova -PakC18,流动相为0.2 %甲醇乙腈溶液-0.1 %磷酸-0.15 %三乙胺(3∶50∶47) ,流速为1.0ml/min ,检测波长为271nm。结果:没食子酸进样量在0.028μg～0.14μg范围内线性关系良好(r=0. 9991) ,平均加样回收率为99.05 % (RSD=0.40 % )。结论:本方法简便、快速、准确、重现性好,可用于蝎子七中没食子酸的含量测定。
OBJECTIVE:To determine the content of gallic acid in viviparous bistort rhizome by HPLC.METHODS:The chromatographic column was Nova-Pak C 18 and the mobile phase was0.2%methanol methyl cyanide solution-0.1%phosˉphoric acid-0.15%triethylamin(3∶50∶47),the flow rate was1.0ml/min and the detection wavelength was271nm.REˉSULTS:It took a good linear relationship when the sample size of gallic acid was within the range of0.028～0.14μg(r=0.9991),the average recovery was99.05%(RSD=4.0%).CONCLUSION:The method is convenient,fast,reproducible,which can be used for the content determination of gallic acid in viviparous bistort rhizome.
出处
《中国药房》
CAS
CSCD
北大核心
2005年第10期780-781,共2页
China Pharmacy
关键词
高效液相色谱法
蝎子七
没食子酸
含量测定
HPLC
Viviparous bistort rhizome
Gallic acid
Content determination
