慢性应激对大鼠认知及脑脊液单胺类神经递质的影响

The impact of chronic stress on cognition and the contents of monoamine transmitters in cerebrospinal fluid of rats

目的探讨慢性应激对大鼠脑单胺类神经递质以及认知功能影响的研究。方法75只SD大鼠随机分为对照组(25只)和慢性应激组(50只),后者以束缚浸水应激方式连续应激21天,每日记录大鼠体重及进食量并进行两组比较,三周后行水迷宫实验、大鼠脑单胺类神经递质测定。结果(1)应激组大鼠体重的增加、进食量较对照组明显减少(P<0.05);(2)水迷宫实验:应激组大鼠的游出时间较对照组明显增加(P<0.05),但错误次数无明显差异(P>0.05)。(3)大鼠脑脊液单胺类神经递质含量(ug/L):5-HT、5-HIAA及MHPG的含量应激组明显低于对照组(P<0.05),NA、DA及HVA在应激组均为升高,但无显著差异(P>0.05)。结论慢性束缚浸水应激三周使大鼠脑脊液中5-HT、5-HIAA及MHPG的含量降低,但对认知功能无明显影响。

Objective: objective:To explore the effects of chronic stress on cognition and contents of monoamine transmitters in cerebrospinal fluid of rats. Methods: 75 Sprague dawley(SD)rats were divided into two groups randomly as normal control group and stress group. Diet, body weight of the rats were observed everyday. 3 weeks later ,water maze test 、the contents of monoamine transmitters in cerebrospinal fluid of rats would be measured. Results:(1)Compared with the control group, the increase of bodyweight、diet of rats in stress group was decreased significantly(P<0.05).(2)There was no difference in the error frequency between two groups in water maze test(P>0.05), while the rats suffered from restraint stress swim slower than control group(P<0.05).(3). There was no difference in the contents of NA、DA and HVA between two groups (P>0.05), while the contents of MHPG、5-HT and 5-HIAA in the stress group are lower significantly than those of in the control group(P<0.05). Conclusion:The contents of MHPG、 5-HT and 5-HIAA in the CSF were obviously decreased, but the changes of the cognition were not remarkable following the chronic stress.