摘要
为了进一步研究A型产气荚膜梭菌C571株的遗传学背景,应用聚合酶链式反应(PCR)技术,从该菌株中扩增出大小为726bp的β2毒素基因(cpb2基因)的部分序列,并将其克隆入pMD18-T载体中。转化至受体菌DH5α后,经Amp/IPTG/X-Gal选择培养,提取质粒,筛选阳性重组克隆。核苷酸序列分析证实,该基因片段与文献报道的β2毒素基因序列一致。
To further study the genetic background of Clostridium perfringens C57-1 type A, a fragment of 726 bp of beta-toxin(cpb2 gene) was amplified by polymerase chain reaction(PCR), and then was cloned into a clone vector pMD18-T. After transformed into E. coli, DH5α, the recombinant clone was selected by Amp/IPTG/X-Gal. DNA sequencing revealed that the cloned DNA fragment was beta2-toxin gene.
出处
《中国兽药杂志》
2005年第5期6-9,共4页
Chinese Journal of Veterinary Drug
