用PCR法检测真菌性鼻窦炎分泌物中的真菌

Detection of Fungi in Secretion of Fungal Sinusitis Using Polymerase Chain Reaction

目的比较真菌内转录片段(ITS)PCR检测方法与培养的敏感性,探讨真菌ITSPCR检测方法的敏感性与特异性及其在真菌性鼻窦炎诊断中的意义。方法取18例临床诊断为真菌性鼻窦炎患者的鼻窦内分泌物,分别做真菌ITSPCR及培养,比较2种方法阳性率的差异及检出真菌的相符率。同时以20例临床诊断为非真菌性慢性鼻窦炎的鼻窦内分泌物为对照。结果18例真菌性鼻窦炎的标本中,ITSPCR方法检测真菌阳性的例数为14例(78%);传统培养的阳性例数为8例(44%)。20例慢性鼻窦炎ITSPCR方法检测阳性例数为1例(5%)。结论ITSPCR方法对于真菌性鼻窦炎的真菌病原快速诊断很有价值,并能通过测序明确感染菌种,是一种高敏感和高特异性的方法。

Objective Polymerase chain reaction (PCR) of internal transcribed spacer region (ITS) is an exquisitely sensitive assay that can detect the DNA of fungal elements. The aim of this study was to compare the sensitivity of conventional culture and techniques using PCR. Methods The samples of secretion were collected from 18 patients diagnosed as fungal sinusitis clinically and 20 cases of control group with chronic sinusitis. Fungal-specific PCR analysis and standard culture were performed on every sample. χ~2 analysis was used to test for statistical differences between the two groups. Results ITSPCR analysis detected fungal DNA in fourteen(78%) patients with chronic fungal sinusitis and eight cases in control group while the positive results of standard cultures were 44%, respectively. Conclusion ITS PCR is more rapid and more sensitive technique than nasal swab culture in detecting the fungi in sinus secretion. The study suggested that it is an effective method in diagnosis of fungal sinusitis.