正常恒河猴经瞳孔温热疗法后眼底组织病理学的实验研究

Histopathological Study of Rhesus Treated by Transpupillary Thermotherapy

目的观察正常恒河猴经瞳孔温热疗法后,不同反应下视网膜的组织病理学变化及其演变过程,了解不同反应对组织的损伤。方法利用经瞳孔温热疗法照射正常恒河猴视网膜。观察照射后1d、1周、2周、1月时阈下反应、弱反应、强反应激光斑在光学显微镜下的病理改变。结果阈下反应光学显微镜下未见病理变化;弱反应光斑病理变化主要在视网膜的外5层;强反应光斑病理变化累及视网膜全层。2周后光斑处的病理改变趋于稳定。结论不同反应的激光斑可以造成不同层次的视网膜损伤。应根据病变的性质选择合适的反应。

Objective To observe the histopathological manifestations and the evolvement processes of different retinal reactions caused by transpupillary thermotherapy (TTT). Methods Transpupillary thermotherapy (TTT) was performed to the retina of normal rhesus monkey. The histopathologic changes of different laser spots no visible reaction, mild reaction and intense reaction at different time 1 d, 1 week, 2 week, and 1 month were observed. Results No change was found in no visible reaction laser spots by light microscope. The histopathologic changes of mild reaction were confined to the outer retina. The histopathologic changes of intense reaction involved the whole layer of retina. The histopathologic changes turned to stable after 2 weeks. Conclusion Different TTT reaction can result in the different extent damage to retina. Suitable reaction should be performed according to the nature of the diseases.