视黄酸对细胞因子诱导的人肺泡Ⅱ型上皮细胞分泌C3及B因子的影响

The effect of retinoic acid on C3 and factor B secretion of human alveolar typeⅡ epithelial cells induced with cytokines

目的:探讨视黄酸(RA)对细胞因子诱导的人肺泡Ⅱ型上皮细胞A549分泌C3及B因子的影响。方法:用ELISA检测TNFα和IL1β诱导的A549细胞培养上清中C3及B因子的水平。用RTPCR分析C3及B因子mRNA的表达。结果:TNFα和IL1β诱导A549细胞分泌C3及B因子具有时间和剂量依赖性。IL6诱导A549细胞分泌C3和B因子的水平是未处理组的4.7、1.4倍,IFNγ诱导A549细胞分泌C3和B因子的水平是未处理组的2.1、1.7倍。RA本身对A549细胞分泌C3及B因子没有影响,但可显著增强TNFα和IL1β诱导的A549细胞分泌C3和B因子及其mRNA的表达,以及IL6和IFNγ诱导的B因子合成。结论:RA可上调TNFα、IL1β、IL6和IFNγ诱导的A549细胞分泌C3及B因子,调节肺局部组织的免疫防御反应,为临床上应用RA和细胞因子防治肺部疾病提供了理论依据。

AIM: To explore the effect of retinoic acid (RA) on C3 and factor B (Bf) secretion by human alveolar type Ⅱ epithelial cell line A549 induced with cytokines. METHODS: The levels of C3 and Bf in culture supernatant of A549 cells induced with cytokines were detected by ELISA. The expression of C3 and Bf mRNAs was analyzed by RT-PCR. RESULTS: TNF-α, IL-1β, IL-6 and IFN-γ could induce the secretion of C3 and Bf of A549 cells. RA alonely had no effect on secretion of C3 and Bf of A549 cells but could notably enhance the secretion of C3 and Bf of A549 cells induced by the cytokines. CONCLUSION: RA can up-regulate the expression of C3 and Bf of A549 cells induced with TNF-α, IL-1β, IL-6 and IFN-γ, and regulate the immunologic defence of local lung tissue, which provides a theoretical basis for prevention and treatment of pulmonary diseases by using RA and cytokines.