摘要
目的:提高抗TNFα单链抗体(scFv)的亲和力。方法:应用错配PCR技术在抗TNFαscFv基因中引入随机突变,再通过DNA交换(shuffling)使引入的点突变进一步重排组合,构建突变噬菌体抗体库。采用硫氰酸盐洗脱法对抗体库进行淘筛。挑选活性得到改良的克隆,用斑点ELISA法及硫氰酸盐洗脱ELISA法评估其亲和力的改善。结果:对PCR错配的突变库筛选未得到亲和力明显改善的抗体变种,经DNA交换进一步构建变种库后,筛选获得1个亲和力提高的克隆,其相对亲和指数为1.37mol/L,较亲本抗体的相对亲和指数0.48mol/L有明显提高。结论:通过错配PCR和DNA交换引入随机突变构建抗体库,可有效地提高scFv的亲和力。
AIM: To improve the affinity of an anti- TNF-α scFv. METHODS: A mutant phage antibody library derived from an anti-TNF-α scFv gene was generated by error-prone PCR. The mutated genes were then subjected to DNA shuffling. Mutants with improved affinity were selected by bio-panning. Affinity improvement of the selected mutants was verified by dot blot ELISA and thiocyanate elusion ELISA. RESULTS: One mutant was obtained with relative affinity index (1.37 mol/L) higher than that of the parent scFv (0.48 mol/L). CONCLUSION: Error-prone PCR plus DNA shuffling is effective in improving the affinity of antibodies.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2005年第4期445-448,共4页
Chinese Journal of Cellular and Molecular Immunology
基金
北京市自然科学基金资助项目(No.5052025)