介入微导丝法建立兔急性大脑中动脉栓塞模型

Making middle cerebral artery occlusion model in rabbits by interventional ways

目的 探讨应用介入放射学方法建立兔急性大脑中动脉栓塞局灶性脑缺血模型的方法,并评价其可靠性。方法 新西兰种大白兔19只,应用股动脉插管技术采用微导管插入一侧颈内动脉,微导丝超选择进入并且栓塞兔大脑中动脉。按缺血时间分为缺血2 h,4 h,6 h组,利用多层螺旋CT灌注成像对各组模型动物的脑缺血状态进行观察,并与红四氮唑(TTC)染色标本对照。结果 缺血2 h组TTC染色未见明显异常。缺血6 h组TTC染色均显示大脑中动脉区域缺血灶形成,CT灌注成像显示为脑血流量下降,脑血容量下降及平均通过时间延长。结论 应用介入微导丝法建立的兔急性大脑中动脉栓塞、局灶性脑缺血模型结果可靠且具有可操作性及重复性。

Objective To develop a new model of middle cerebral artery occlusion in rabbits by interventional ways. Methods Femoral artery cannulation was performed in nineteen New Zealand rabbits. The microcatheter was used to insert into internal carotid artery, then microfilament was used to embolize middle cerebtal artery superselectively. All rabbits were divided into three groups according to different ischemic time 2 hours, 4 hours and 6 hours. Multislice CT perfusion imaging and 2,3,5-triphenylterrazolium chloride (TTC) staining were used to evaluate the level of ischemia. Results TTC staining showed that there was no obvious infarction in ischemic time 2 hours group. There had definite infarction in territory of MCA in ischemic time 6 hours group. Obviously low cerebral blood flow, low cerebral blood volume,prolonged mean transit time were found in CT perfusion mappings. Conclusion The middle cerebral artery occlusion model of rabbit made by interventional ways is stable and reproducible.