p14^(ARF)基因变异及其产物表达与甲状腺肿瘤的关系

Study of alteration of gene p14^(ARF) and expression of its product and the relationship between gene p14^(ARF) and thyroid tumors

目的探讨甲状腺肿瘤p14ARF基因变异及其产物的表达与甲状腺肿瘤发生发展的关系。方法应用双重PCR和免疫组化SP法分别检测20例甲状腺腺瘤和28例甲状腺癌组织中p14ARF基因纯合性缺失及其蛋白表达。28例甲状腺癌中,11例甲状腺滤泡癌、12例乳头状癌、4例髓样癌、1例未分化癌。结果1p14ARF基因在甲状腺腺瘤及癌中的纯合性缺失率分别为5.0%、42.9%,二者有显著性差异(P<0.01)。2p14ARF蛋白在甲状腺腺瘤及癌中的阳性率分别为90.0%、35.7%,二者有显著性差异(P<0.05)。结论1甲状腺肿瘤p14ARF基因纯合性缺失是其蛋白表达失活的重要机制之一,与甲状腺肿瘤的发生发展密切相关。2p14ARF可能是甲状腺肿瘤的分子标志物之一,可作为鉴别甲状腺腺瘤与滤泡癌的辅助指标。

Objective To study the relationship between gene p14~ARF and thyroid tumors by measuring alteration of gene p14~ARF and expression of its product.Methods 20 thyroid adenomas and 28 thyroid carcinomas (11 folicular carcinomas,12 papillary carcinomas,4 medullary carcinomas and 1 undifferentiated carcinoma) were selected and their alteration of p14~ARF and expression of its product,including homozygous deletion were detected by differential PCR and protein expression were deteeted by immunohis to chemistry.Results ① Homozygous deletion rate of p14~ARF in thyroid adenomas and carcinomas were 5.0%,42.9% respectively,there was significant difference between adenomas and carcinomas (P<0.01).② Positive rate of protein p14~ARF in thyroid adenomas and carcinomas were 90.0%、 35.7% respectively,there also was significant difference between them(P<0.05).Conclusions ①In thyroid tumors,homozygous deletion of p14~ARF is one of important mechanisms of loss of protein and is related to the happening and development of thyroid tumors.②p14~ARF may be one of molecular markers of thyroid tumors and can be used as an assistant index to distinguish thyroid folicular carcinoma from adenoma.