HPLC测定炒决明子中总大黄酚的含量

Quantitative analysis of total chrysophanol in the processed seeds of Cassia obtusifolia by HPLC

目的:测定炒决明子中总大黄酚的含量,为决明子饮片的质量控制提供依据。同时对不同产地的生、炒决明子中大黄酚成分的含量进行测定。方法:采用高效液相色谱法,以甲醇0. 1 磷酸水溶液(85∶15)为流动相, 254nm,流速1. 0mL·min-1下以KromasilC18色谱柱检测。结果:总大黄酚含量为0. 196 4 ,加样回收率为96. 95 。炒制后游离大黄酚含量增加,总大黄酚及结合大黄酚含量减少。结论:该方法可信,可作为决明子饮片质量标准之一,为最终确定决明子炮制的最佳工艺奠定基础。

AIM: To analyze the total chrysophanol contents in the processed seeds of Cassia Tora and the contents of chrysophanol in the crude and processed seeds from different districts are compared. METHODS: HPLC with a Kromasil C 18 column was used and MeOH 0.1%H 3PO 4(85∶15) used as the mobile phase. The flow rate was 1.0 mL·min -1 and detection wavelength was 254 nm. RESULTS: The contents of total chrysophanol was 0.196 4% and the average recovery was 96.95%. The content of free chrysophanol was increased after processing, total and combined chrysophanol were decreased. CONCLUSION: The method is reliable of controlling the quality of Cassia obtusifolia and can be used for further studies in the processing of Cassia obtusifolia.