Survivin在儿童急性白血病中的表达和P53的相关性及其临床意义

Expression of Survivin in pediatric acute leukemia and its relationship with expression of P53 and clinical significance.

目的探讨Survivin在儿童急性白血病(AL)中的表达及其与抑癌基因P53的相关性,同时观察Sur vivin与急性淋巴细胞白血病(ALL)临床分型的关系。方法应用免疫组织化学链霉亲和素生物素过氧化物酶复合物(SABC)方法,检测华中科技大学同济医学院附属同济医院儿科2004年1～7月收治的37例儿童AL骨髓细胞Survivin和P53的表达,对照组为10例非恶性血液病且骨髓正常患儿。结果在37例AL患儿中,Survivin蛋白表达阳性率为35.14%,P53表达阳性率为32.43%,显著高于正常骨髓组织(P均<0.05),Survivin和P53表达呈强相关性(P<0.05),Survivin在高危(HR)ALL患儿的表达为46.15%,在标危(SR)ALL患儿的表达为0,差异有显著性意义(P<0.05)。结论(1)Survivin在儿童AL中异常表达,且与ALL的临床分型有关,提示其在判定儿童ALL预后中有意义;(2)抑癌基因P53的失活与Survivin的表达可能在AL发生中起协同作用。

Objective To investigate the expression of Survivin in pediatric acute leukemia and its relationship with expression of P53 and clinical significance.Methods The expression of Survivin and P53 proteins were detected by immunohistochemical assay in 37 acute leukemia children.The control included 10 children with normal bone marrow.Results Survivin protein expression rate was 35.14% and P53 protein expression rate was 32.43% in 37 AL children.Both of them were significantly higher than that in control group (P<0.05).The expression of Survivin and P53 being strongly related (P<0.05).The expression rate of Survivin was 46.15% in HR ALL and 0% in SR ALL,the difference was statistically significant(P<0.05).Conclusion (1)Survivin protein expression rate is abnormal and is associated with the clinical typing of ALL.It may play an important role in the prognosis of ALL.(2)Expression of Survivin and de-activation of anti-oncogene P53 might play synergetic roles in the process of genesis of AL.