同时检测抗SSA和抗RNP在诊断结缔组织病中的意义

Clinical significance of combined determinaton of antiSSA and antiRNP in diagnosis of connective tissue disease

目的探讨抗SSA和抗RNP在结缔组织病中出现的机率。方法采用双免疫扩散法(ID)和免疫印迹法(IBT)同时检测抗SSA和抗RNP。结果用ID法检测系统性红斑狼疮(SLE)患者抗SSA阳性率远高于抗RNP(P<0.05),混合性结缔组织病患者中抗RNP阳性率l00%高于抗SSA的19%(P<0.001),结缔组织病患者中抗SSA阳性率82.8%亦远高于抗RNP的14.1%(P<0.05)。结论ID法比IBT法检测抗SSA较全面,使用ID法和IBT法同时检测抗SSA和抗RNP,可以提高临床诊断的正确性,减少漏诊。

Objective To assess the significance of combined determination of antiSSA and antiRNP in diagnosis of connective tissue disease.Methods The antiSSA and antiRNP were measured by immunodiffusion (ID) and immunoblotting technique (IBT) at the same time.Results The positive rate of antiSSA was higher than that of antiRNP in systemic lupus erythematosus(SLE)(P<0.05).The positive rate of antiRNP was 100% in mixed connective tissue disease and higher than that of antiSSA(P<0.001).The positive rate of antiSSA was 82.8% ,and higher than that of anti RNP(14.1%,) in connective tissue disease(P<0.05).Conclusion The methed of ID is better than IBT to measure antiSSA, combined antiSSA and antiRNP by ID and IBT at the same time can improve clinical diagnosis,to avoid misdiagnosis.