清开灵、复方丹参、黄芪3种注射液抗大鼠脑缺血的比较研究

Effects of Qingkailing, Fufangdanshen and Huangqi Injection in Treatment of Acute Cerebral Infarction (ACI)

目的比较清开灵、复方丹参、黄芪注射液抗大鼠急性脑缺血的作用。方法运用线栓法造成大鼠局灶性脑缺血,造模后2h分别腹腔注射3种药物2.5mL/kg,72h后取材,观测指标为梗死体积比、脑水肿比。通过测定加药物血浆的血小板聚集率,比较3种药物对血小板聚集的影响。结果清开灵、复方丹参、黄芪注射液给药组均能有效降低脑梗死体积比,与模型组比较,P<0.05或P<0.01;复方丹参、黄芪注射液组能降低脑水肿比,与模型组比较,P<0.05或P<0.01。3种注射液终浓度为50、25、12.5μL/mL,均能降低血小板的聚集率,其中,黄芪注射液作用强于清开灵、复方丹参注射液。结论清开灵、复方丹参、黄芪注射液均有抗大鼠脑缺血和血小板聚集作用。

Objective To observe the differential effects of Qingkailing, Fufangdanshen and Huangqi injection in treatment of acute cerebral infarction. Methods After MCAO-induced focal brain ischemia, those three kinds of injection were separately injected ip. Brain infarct area and brain piece area were measured with computer-assisted imaging after 72 hours. Observing indexs are infract volume rate and oedeme rate. Determining platelet assembling rate outside body, we made compare in the function of anti-platelet among three kinds of medicine. Results Qingkailing, Fufangdanshen and Huangqi efficiently reduced brain infarct volume rate, and the latter two injections decreased oedeme rate. Three kinds of injections all reduced platelet assembling rate, and Huangqi was better than others in the function. Conclusion Qingkailing, Fufangdanshen and Huangqi can efficiently treat ACI.