HPLC法测定精宝丸中齐墩果酸的含量

Determination of Oheanolic Acid in Jingbao Pills by HPLC

目的建立精宝丸中齐墩果酸的含量测定方法。方法采用HPLC,选用ODS色谱柱,以甲醇-0.4%冰醋酸溶液(87∶13)为流动相,检测波长220nm。结果此方法在16.0～160.0μg/mL范围内线性关系良好(r=0.99996),平均回收率为99.92%,RSD=1.21%(n=5)。结论此方法简便、灵敏、准确,可用于精宝丸的质量控制。

Objective To establish the content determination method of oheanolic acid in Jingbao Pills by HPLC. Methods HPLC was performed to determine oheanolic acid on ODS column. The mobile phase was methanol-0.4% acetic acid (87∶13). Detecting wavelength was 220 nm. Results The linearity of Oheanolic acid was in the range of 16.0~160.0 μg/mL (r =0.99 996), and the average recovery was 99.92%, RSD=1.21%. Conclusion This method was simple, sensitive, accurate and available for the quality control of Jingbao Pills.