凝血酶原G20210A与乳腺癌、胃癌的相关性

The Association of the Prothrombin Gene Mutation(20210G/A) with Breast Cancer and Gastric Carcinoma

目的对正常人及乳腺癌、胃癌患者凝血酶原20210位点多态性进行分析,探讨20210A与上述疾病的关系。方法采用聚合酶链反应(PCR)、限制性酶切及电泳分型等方法,对疾病组和对照组的凝血酶原基因20210位点的多态性进行分析。结果①正常人、乳腺癌、胃癌患者盅凝血酶原20210A基因频率分别是0.98%、0.94%、1.47%;②乳腺癌组20210A基因频率(0.0094)与正常女性对照组(0.0103)比较差异无显著性(P>0.05)。胃癌组20210A基因频率(0.0147)与正常对照组(0.0098)比较差异亦无显著性(P>0.05)。结论正常女性与乳腺癌、正常人与胃癌20210G/A基因频率分布均相似。

Objective To determine the distribution of gene type of 20210G/A of prothrombin and whether it might be implicated in breast cancer and gastric carcinoma.Methods Peripheral blood were collected from 254 normal controls, 53 patients with breast cancer and 34 patients with gastric carcinoma polymerase chain reaition (PCR) combined with restriction enzyme digestim,electrophoresis were med to analyze.the 20210 G/A allele polymorphism in the prothrombin gene.Results ① The 20210A allele frequencies of prothrombin gene in control、 Breast cancer and gastric carcinoma were 0.98%, 0.94%, 1.47% respectively,②The gene frequencies of 20210A of prothrombin gene on breast cancer(0.0094) and gastric carcinoma(0.0147) are not significantly higher than that of control subjects.Conclusions The allele frequency distribution of 20210G/A did not differ in patients with breast cancer or gastric carcinoma from that in contrals,suggesting that this allele should not be considered a major risk factor for breast cancer and carcinoma gastric.