茶树（Camellia　Sisensis　O．　Ktze）叶片培养的器官发生

Organogenesis of Leaf Culture of Camellia sinensis O. Ktze.

以叶片为外植体，采用ＭＳ基本培养基，附加１～５ｍｇ／Ｌ的６－苄基嘌呤（６－ＢＡ）、２ｍｇ／Ｌ吲哚乙酸（ＩＡＡ）、３ｍｇ／Ｌ赤霉素（ＧＡ３），０．２～０．３％活性炭诱导愈伤组织。并用ＭＳ或ＥＲ为基本培养基，附加０．５～５ｍｇ／Ｌ６－苄基嘌呤（６－ＢＡ），０．５ｍｇ／Ｌ吲哚丁酸（ＩＢＡ）诱导胚状体和器官，进一步形成小植株。采取ＭＳ加５～８ｍｇ／Ｌ细胞分裂素和０．５～１ｍｇ／Ｌ的细胞生长素，每天光照１０小时便形成“丛生叶”，植株矮小；若上列激素的配比接近时，每天光照１２～１４ｈ，即打破丛生叶形态，节间抽长形成正常的互生叶小植株。

n this study, leaves of Camellia Sinensis O. Ktze. were cultured as explants, and thecallus was obtained in MS medium supplemented with 6 - BA (1 - 5mg/L) , IAA(2mg/L. GA3 (3mg,L) and activated carbon (0.2-0.3%). Embryogenesis and organogenesiswere induced in the MS or ER medium added with 6-BA (0.5-5.omg/L), IBA (0.5-5.0ml/L)and finally complete plantlets were formed. Caespitose leaves were formed in the MSmedium supplemented with cytokin (5-8mg/L) and auxin (0.5-l.0mg/L) under 10 hr dailyphotoperiod of l, 500 lux light intensity, and the plantlets were small and short. If theproportion of auxin to cytokin is close to l: l, the caespitose state was broken under12-14 hr daily photoperiod of 2, 000 lux light intensity, the internodeswere stretchedand the leaflets with alternate leaves were formed.