摘要
目的探讨转录因子 GATA-3在哮喘大鼠模型肺组织中的表达及其气道炎症中的作用,并观察地塞米松对 GA-TA-3的表达及气道炎症的影响。方法 30只 Wista 大鼠随机分为3组(正常对照组,哮喘组,地塞米松治疗组),常规方法制作哮喘模型,并用地塞米松进行治疗,用免疫组化检测肺组织 GATA-3的表达,用 HE 染色的方法观察肺组织气道炎症的改变。结果与正常对照组比,哮喘组大鼠肺组织中 GATA-3的表达明显增加(P<0.05),同时气道粘膜和周围肺组织内可见明显的嗜酸粒细胞浸润。地塞米松能明显降低大鼠肺组织中 GATA-3的表达及气道炎症反应。结论转录因子 GATA-3在哮喘大鼠肺组织中的过度表达可能参与了哮喘气道炎症的发生及病理过程。
Objective To observe the expression of GATA-3 in the pulmonary tissue and its roles in the airway inflammation of asthmate models of Wistar rat.Methods Wistar rats were selected to make the model of asthma.They were divided into three groups(asthma group,nor- mal control group,dexamethasone group).The expression of GATA-3 were evalued by immunohistochemistry,and HE staining were used to detect the inflammatory cell infiltration and the inflammatory state of airway.Results The number of GATA-3 positive cells,numerical density on area,and average optical density(OD)value are significantly higher in pulmonary tissue of asthma group than that of normal control group, there am significant differences between the two groupe(P<0.01,respectively).And dexamethasone could reduce significantly the expression of GATA-3 and the inflammatory reaction in pulmonary tissue.Conclusion The over-expression of transcription factor GATA-3 might con- tribute to the pathology and the inflammatory reaction of airway in pulmonary tissue.
出处
《医学研究通讯》
2005年第6期42-44,共3页
Bulletin of Medical Research
