dl-四氢巴马汀抗大鼠胃溃疡作用

Effect of dl-tetrahydropalmatine on rat gastric ulcer

目的研究dl-四氢巴马汀(dl-THP)对大鼠急、慢性溃疡的保护作用,并初步分析其机制。方法胃黏膜损伤由吲哚美辛、束缚应激、结扎幽门和胃浆膜下注入醋酸引起。dl-THP15,30,45mg·kg-1灌胃,1日2次,共5次。测定大鼠胃溃疡数目、面积、胃酸分泌、胃蛋白酶活性以及胃黏膜血流。结果dl-THP15,30,45mg·kg-1能抑制大鼠胃黏膜损伤,且呈剂量依赖性;dl-THP30mg·kg-1对大鼠慢性胃溃疡的抑制率为75.8%,与雷尼替丁30mg·kg-1的67.7%相仿。dl-THP可增加胃黏膜血流,但对胃酸和胃蛋白酶分泌无影响。结论dl-THP具有明显抗实验性胃溃疡作用,可能与其增加胃黏膜血流有关,亦可能部分通过阻滞胃黏膜DA受体而发挥作用。

OBJECTIVE: To study the effect of dl-tetrahydropalmatine (dl-THP) on various rat gastric ulcers and analyze its possible mechanism. METHODS: The gastric mucosal damage was induced by pyloric ligation, restraint, indomethacin and acetic acid in rats, dl-THP 15, 30 and 45 mg&middotkg-1 was given ig bid for 5 doses. Then the number of gastric ulcers or the gastric ulcer area, the secretion of gastric juice, hydrochloric acid and pepsin activity were determined respectively. The stomach blood flow in normal and dl-THP treated rats was also examined. RESULTS: dl-THP 15, 30, 45 mg&middotkg -1 protected the gastric mucosa against the damages with a dose-dependent manner. In chronic gastric ulcer model, the inhibitory rates of gastric ulcer of dl-THP and ranitidine 30 mg&middotkg-1 were 75.8% and 67.7%, respectively. However, the secretions of gastric juice, HCl, and pepsin by gastric mucosa were not influenced by dl-THP. Moreover, dl-THP increased stomach blood flow significantly. CONCLUSION: dl-THP protected various experimental gastric ulcers by increasing stomach blood flow and maybe partly by antagonizing gastric DA receptors.